La Yongfu, Liu Qiuyue, Zhang Liping, Chu Mingxing
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Animals (Basel). 2019 Jul 9;9(7):432. doi: 10.3390/ani9070432.
, , and have been extensively studied as candidate genes because of their great influence on the reproductive traits of animals. However, little is known about the association between polymorphisms of the , , and genes and litter size in Small-Tail Han sheep. In this study, the expression levels of , , and in HPG (hypothalamic-pituitary-gonadal) axis tissues of polytocous and monotocous Small-Tail Han sheep were analyzed by qPCR. To better understand the effects of four single nucleotide polymorphisms (SNPs) comprising of g.70067210 T > C in , g.25350431 C > T and g.25360220 T > C in , and g.14413132 C > T in , a population genetic analysis was conducted using data obtained from genotyping in 728 sheep from seven breeds. The results indicated that all genes included in this study were differentially expressed in the pituitary and uterus of polytocous and monotocous Small-Tail Han sheep ( < 0.05). The associations of these four SNPs and the mutation with litter size in 384 Small-Tail Han sheep were analyzed, therefore, and it was found that both g.70067210T > C and the mutation were significantly associated with litter size ( < 0.05). The linear regression analysis of the association of multiple markers ( and g.70067210 T > C in ) with litter size indicated that homozygous ewes carrying the BB/TT genotype had larger litter size than any ewes with any other genotype. In conclusion, the SLC5A1 SNPs significantly affect litter size in sheep and are useful as genetic marker for litter size.
由于对动物繁殖性状有重大影响，[相关基因名称]已被广泛研究作为候选基因。然而，关于[相关基因名称]基因多态性与小尾寒羊产羔数之间的关联知之甚少。本研究通过qPCR分析了多胎和单胎小尾寒羊下丘脑-垂体-性腺（HPG）轴组织中[相关基因名称]的表达水平。为了更好地了解由[基因名称1]中g.70067210 T>C、[基因名称2]中g.25350431 C>T和g.25360220 T>C以及[基因名称3]中g.14413132 C>T组成的四个单核苷酸多态性（SNP）的影响，利用从七个品种的728只绵羊基因分型获得的数据进行了群体遗传分析。结果表明，本研究中包含的所有基因在多胎和单胎小尾寒羊的垂体和子宫中均有差异表达（P<0.05）。因此，分析了这四个SNP和[相关突变名称]突变与384只小尾寒羊产羔数的关联，发现g.70067210T>C和[相关突变名称]突变均与产羔数显著相关（P<0.05）。多个标记（[基因名称1]中的[具体标记名称]和g.70067210 T>C）与产羔数关联的线性回归分析表明，携带BB/TT基因型的纯合母羊产羔数比任何其他基因型的母羊都大。总之，SLC5A1 SNP显著影响绵羊的产羔数，可作为产羔数的遗传标记。
