Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei, China.
Reprod Domest Anim. 2020 Sep;55(9):1145-1153. doi: 10.1111/rda.13753. Epub 2020 Jul 7.
TGF-β induced factor homeobox 1 (TGIF1) and splicing factor 1 (SF1) are important for mammalian reproduction; however, the effects of these genes on litter size in sheep remain unexplored. In this study, we genotyped 768 ewes from seven sheep breeds at two loci: g.37871539C>T, a synonymous mutation of TGIF1; and g.42314637T>C, a 3'UTR variant of SF1. Our analysis of polymorphism revealed only two genotypes at locus g.37871539C>T in TGIF1, with most sheep populations being moderately polymorphic (0.25 < PIC < 0.5) at this site. In contrast, most breeds exhibited low polymorphism (PIC ≤0.25) at the SF1 locus g.42314637T>C. The association analysis revealed that a synonymous mutation at g.37871539C>T in TGIF1 was highly associated with litter size in Small Tail Han sheep, in which it causes a significant decrease in litter size. Conversely, while the SF1 3'UTR variant g.42314637T>C was also highly associated with litter size in sheep, it causes a significant increase in the number of litter size. Combined, these data provide valuable information regarding candidate genetic markers for sheep breeding programs.
TGF-β 诱导因子同源盒 1（TGIF1）和剪接因子 1（SF1）对哺乳动物的繁殖至关重要；然而，这些基因对绵羊产仔数的影响仍未被探索。在这项研究中，我们在两个基因座 g.37871539C>T（TGIF1 的同义突变）和 g.42314637T>C（SF1 的 3'UTR 变体）对来自七个绵羊品种的 768 只母羊进行了基因分型。我们对多态性的分析表明，TGIF1 基因座 g.37871539C>T 只有两种基因型，大多数绵羊群体在该位点表现出中度多态性（0.25<PIC<0.5）。相比之下，大多数品种在 SF1 基因座 g.42314637T>C 处表现出低多态性（PIC≤0.25）。关联分析表明，TGIF1 基因座 g.37871539C>T 的同义突变与小尾寒羊的产仔数高度相关，它导致产仔数显著减少。相反，虽然 SF1 3'UTR 变体 g.42314637T>C 也与绵羊的产仔数高度相关，但它导致产仔数显著增加。综合这些数据，为绵羊育种计划提供了有价值的候选遗传标记信息。
