Seo Bo-Young, Choi Mi-Joo, Kim Ji-Sang, Park Eunju
Department of Food and Nutrition, Changshin University, Gyeongnam 51352, Korea.
Department of Food and Nutrition, Kyungnam University, Gyeongnam 51767, Korea.
Prev Nutr Food Sci. 2019 Jun;24(2):128-135. doi: 10.3746/pnf.2019.24.2.128. Epub 2019 Jun 30.
The aim of this study was to compare ginsenosides profiles, and antioxidant, antiproliferative, and antigenotoxic activities of ginseng extract derived from fine and main roots. The result of the analysis showed a higher total content of ginsenoside in fine roots than in main roots; differences in levels between the different extracts were also confirmed. The oxygen radical absorbance capacity (ORAC) assay showed that HO main root extract had a significantly higher activity than that from fine roots. MeOH and HO extracts from the fine and main roots also exhibited stronger cellular antioxidant capacity 2,2'-azobis(2-amidinopropane) dihydrochloride-induced oxidative stress in HepG2 cells compared with the positive control. Through calculating the half-maximal inhibitory concentration values, the cytotoxicity of the main root extracts were ranked as follows: MeOH (6.1±1.2 μg/mL)> HO (6.6±0.1 μg/mL)> ethanol (10.4±0.6 μg/mL); however, the cytotoxicity of all fine root extracts did not significantly differ. All the fine root extracts showed an inhibitory capacity against 4-hydroxynonenal-induced DNA damage, however only the MeOH extract of the main root showed a decrease in DNA damage. All three solvent extracts from the fine roots reduced DNA damage more in the HO-treated group, whereas only the MeOH and HO extracts of the main roots produced a significant reduction. Levels of Rg3 ginsenoside were positively correlated with indices of the ORAC value, and total ginsenoside contents showed a negative correlation with DNA damage induced by HO. This study suggests that ginseng and the extraction solvent both affect levels of ginsenoside. Furthermore, the antioxidant potency of ginseng can be attributed to the content of some ginsenosides.
本研究旨在比较细根和主根人参提取物的人参皂苷谱、抗氧化、抗增殖和抗遗传毒性活性。分析结果表明，细根中人参皂苷的总含量高于主根；不同提取物之间的含量差异也得到了证实。氧自由基吸收能力（ORAC）测定表明，主根提取物的活性显著高于细根提取物。与阳性对照相比，细根和主根的甲醇提取物和水提取物在HepG2细胞中也表现出更强的细胞抗氧化能力，可抵抗2,2'-偶氮二异丁脒二盐酸盐诱导的氧化应激。通过计算半数抑制浓度值，主根提取物的细胞毒性排序如下：甲醇提取物（6.1±1.2 μg/mL）>水提取物（6.6±0.1 μg/mL）>乙醇提取物（10.4±0.6 μg/mL）；然而，所有细根提取物的细胞毒性没有显著差异。所有细根提取物均表现出对4-羟基壬烯醛诱导的DNA损伤的抑制能力，然而只有主根的甲醇提取物表现出DNA损伤的减少。细根的所有三种溶剂提取物在过氧化氢处理组中对DNA损伤的减少作用更大，而只有主根的甲醇提取物和水提取物产生了显著的减少。人参皂苷Rg3的含量与ORAC值指标呈正相关，总人参皂苷含量与过氧化氢诱导的DNA损伤呈负相关。本研究表明，人参和提取溶剂都会影响人参皂苷的含量。此外，人参的抗氧化能力可归因于某些人参皂苷的含量。
