Department of Plant Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari 4818166996, Iran.
National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran 14965161, Iran.
Genes (Basel). 2023 Mar 20;14(3):753. doi: 10.3390/genes14030753.
Plants have acquired sets of highly regulated and complex signaling pathways to respond to unfavorable environmental conditions during evolution. Calcium signaling, as a vital mechanism, enables plants to respond to external stimuli, including abiotic and biotic stresses, and coordinate the basic processes of growth and development. In the present study, two calcium sensor families, CBL and CIPK, were investigated in a halophyte plant, , with a comprehensive analysis. Here, six genes, and twenty genes were studied. The analysis of the gene structure and conserved motifs, as well as physicochemical properties, showed that these genes are highly conserved during evolution. The expression levels of genes and genes were evaluated under salt stress in leaf and root tissue. Based on the real-time RT-PCR results, the gene family had a higher variation in mRNA abundance than the gene family. genes were found to have a higher abundance in leaves than in roots. The results suggest that the correlation between genes and is tissue-specific, and different correlations can be expected in leaves and roots. Based on these correlations, AlCIPK3.1-AlCBL4.1 and AlCIPK1.2-AlCBL4.4 can be co-expressed in the root tissue, while AlCBL10 has the potential to be co-expressed with AlCIPK5, AlCIPK26, and AlCIPK12.3 in the leaf tissue. Our findings reveal valuable information on the structure and function of calcium sensor families in a halophyte plant, that can be used in future research on the biological function of CBLs and CIPKs on salt stress resistance.
植物在进化过程中已经获得了一系列高度调控和复杂的信号通路，以应对不利的环境条件。钙信号作为一种重要的机制，使植物能够对外界刺激（包括生物和非生物胁迫）做出反应，并协调生长和发育的基本过程。在本研究中，对一种盐生植物 中的钙传感器家族 CBL 和 CIPK 进行了全面分析。在这里，研究了六个 基因和二十个 基因。通过对基因结构和保守基序以及理化性质的分析，表明这些基因在进化过程中高度保守。在叶片和根组织中，评估了 基因和 基因在盐胁迫下的表达水平。基于实时 RT-PCR 结果，发现 基因家族的 mRNA 丰度变化高于 基因家族。在叶片中发现 基因的丰度高于根。这些结果表明， 基因与 之间的相关性是组织特异性的，在叶片和根中可以预期不同的相关性。基于这些相关性，AlCIPK3.1-AlCBL4.1 和 AlCIPK1.2-AlCBL4.4 可以在根组织中共表达，而 AlCBL10 有可能与 AlCIPK5、AlCIPK26 和 AlCIPK12.3 在叶组织中共表达。我们的研究结果揭示了盐生植物中钙传感器家族结构和功能的有价值信息，可用于研究 CBLs 和 CIPKs 在耐盐性方面的生物学功能。
