免疫球蛋白VH基因由T细胞与一个新的5'外显子一起转录。
Immunoglobulin VH genes are transcribed by T cells in association with a new 5' exon.
作者信息
Baer R, Forster A, Lavenir I, Rabbitts T H
机构信息
Medical Research Council Laboratory of Molecular Biology, Cambridge, England.
出版信息
J Exp Med. 1988 Jun 1;167(6):2011-6. doi: 10.1084/jem.167.6.2011.
Abstract
We previously detected mRNAs in a number of human T cell lines with a probe from within the Ig VH gene locus. We now show these mRNAs consist of Ig VH genes expressed in T cells. In one human T cell line, two RNA species have been studied and found to come from transcripts of unrearranged VH segments in which the leader exon, normally associated with VH transcripts in B cells, is replaced by a novel 5' exon (ET) not encoding a hydrophobic leader peptide. In genomic DNA, this new ET exon is adjacent to a pseudo-VH gene that has not been observed in mature mRNA. This implies that RNA splicing controls association of the new exon with the expressed VH segments. Hence, VH transcription does indeed occur in T cells, but is qualitatively different from that in B cells.
摘要
我们之前用来自免疫球蛋白重链可变区（Ig VH）基因座内的探针在多种人类T细胞系中检测到了mRNA。我们现在表明，这些mRNA由在T细胞中表达的Ig VH基因组成。在一种人类T细胞系中，对两种RNA种类进行了研究，发现它们来自未重排的VH区段的转录本，其中通常与B细胞中VH转录本相关的前导外显子被一个不编码疏水前导肽的新的5'外显子（ET）所取代。在基因组DNA中，这个新的ET外显子与一个在成熟mRNA中未观察到的假VH基因相邻。这意味着RNA剪接控制了新外显子与表达的VH区段的关联。因此，VH转录确实在T细胞中发生，但在性质上与B细胞中的不同。
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