Mendonça Pedro Henrique Braz, da Rocha Raphael Francisco Dutra Barbosa, Moraes Julliane Brito de Braz, LaRocque-de-Freitas Isabel Ferreira, Logullo Jorgete, Morrot Alexandre, Nunes Marise Pinheiro, Freire-de-Lima Celio Geraldo, Decote-Ricardo Debora
Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Rio de Janeiro, Brazil.
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Front Immunol. 2017 May 31;8:604. doi: 10.3389/fimmu.2017.00604. eCollection 2017.
is an obligatory intracellular protozoan parasite, and it is the etiological agent of Chagas' disease that is endemic in the Americas. In addition to humans, a wide spectrum of mammals can be infected by , including dogs. Dogs develop acute and chronic disease, similar to human infection. can infect almost all cell types and after cell invasion, the metacyclics trypomastigotes localize in the cytoplasm, where they transform into amastigotes, the replicative form of in mammals. After amastigote multiplication and differentiation, parasites lyse host cells and spread through the body by blood circulation. In this work, we evaluated the ability of to infect a canine macrophage cell line DH82 compared with RAW264.7, a murine tissue culture macrophage. Our results have shown that the is able to infect, replicate and differentiate in DH82 cell line. We observed that following treatment with LPS and IFN-γ DH82 cells were more resistant to infection and that resistance was not related reactive oxygen species production in our system. In this study, we also found that DH82 cells became more susceptible to infection when cocultured with apoptotic cells. The analysis of cytokine production has showed elevated levels of the TGF-β, IL-10, and TNF-α produced by -infected canine macrophages. Additionally, we demonstrated a reduced expression of the MHC class II and CD80 by infected DH82 cell line.
是一种 obligatory 细胞内原生动物寄生虫，是美洲流行的恰加斯病的病原体。除人类外，包括狗在内的多种哺乳动物都可被 感染。狗会出现与人类感染相似的急性和慢性疾病。 几乎可以感染所有细胞类型，细胞入侵后，循环后期锥鞭毛体定位于细胞质中，在那里它们转化为无鞭毛体，这是 在哺乳动物中的复制形式。无鞭毛体增殖和分化后，寄生虫裂解宿主细胞并通过血液循环在体内传播。在这项工作中我们评估了 与小鼠组织培养巨噬细胞 RAW264.7 相比感染犬巨噬细胞系 DH82 的能力。我们的结果表明 能够在 DH82 细胞系中感染、复制和分化。我们观察到用 LPS 和 IFN-γ 处理后，DH82 细胞对感染更具抗性，并且这种抗性与我们系统中活性氧的产生无关。在本研究中，我们还发现与凋亡细胞共培养时，DH82 细胞对 感染更敏感。细胞因子产生分析表明，受 感染的犬巨噬细胞产生的 TGF-β、IL-10 和 TNF-α 水平升高。此外，我们证明受感染的 DH82 细胞系中 MHC II 类和 CD80 的表达降低。
“obligatory”这个词在医学语境中可能不太常见，这里直接保留英文未翻译，因为可能是特定专业术语或拼写有误，若有更准确的背景信息，翻译会更完善。 这里的“ ”指代不明，原文可能有信息缺失。 整体译文在部分专业术语翻译上可能因原文信息问题不够精准，但已尽量按照要求准确翻译。
