Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan.
Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan.
Food Chem Toxicol. 2019 Oct;132:110696. doi: 10.1016/j.fct.2019.110696. Epub 2019 Jul 22.
Ochratoxin A (OA), the secondary metabolite of certain Aspergillus and Penicillium species, is one of the potent biological immune-suppressor. The present study was designed to explore the in-vivo efficacy of Trichosporon mycotoxinivorans (TR); yeast strain isolated from the hindgut of the termite Mastotermes darwiniensis, against the immunotoxicity of OA in broiler birds. For this purpose, broiler chicks were offered diet added with TR (0.5, 1.0 or 2.0 g/kg feed) and/or OA (0.15, 0.3 or 1.0 mg/kg feed) for 42 days. Dietary OA at all levels, resulted in significant reduction (p ≤ 0.05) in the immune response of broiler birds as recorded by vacuolation and darkly stained pyknotic nuclei in bursa of Fabricius and thymus, humoral immune responses to sheep red blood cells (SRBC), in-vivo lymphoproliferative response to Phytohemagglutinin-P (PHA-P) and mononuclear phagocytic system function assay. Addition of TR in broiler diet significantly reduced (p ≤ 0.05) the immunotoxicity of OA at 0.15 and 0.30 mg/kg; however, against higher dietary level of OA (1.0 mg/kg), a partial protection was observed. Feeding TR alone had no immunomodulatory effect at any of tested level. Dietary addition of TR is proposed as an approach to combat the OA mediated immunological damages in broiler birds.
赭曲霉毒素 A（OA）是某些曲霉属和青霉属物种的次生代谢产物，是一种有效的生物免疫抑制剂。本研究旨在探索从白蚁后肠分离的毛孢子菌（TR）酵母菌株对肉鸡中 OA 免疫毒性的体内疗效。为此，肉鸡饲喂添加 TR（0.5、1.0 或 2.0 g/kg 饲料）和/或 OA（0.15、0.3 或 1.0 mg/kg 饲料）的饲料 42 天。日粮 OA 各水平均导致肉鸡的免疫反应显著降低（p≤0.05），表现为法氏囊和胸腺空泡化和深染固缩核，对绵羊红细胞（SRBC）的体液免疫反应，植物血凝素-P（PHA-P）体内淋巴细胞增殖反应和单核吞噬细胞系统功能测定。在肉鸡日粮中添加 TR 可显著降低（p≤0.05）0.15 和 0.30 mg/kg OA 的免疫毒性；然而，对较高水平的日粮 OA（1.0 mg/kg），则观察到部分保护作用。单独饲喂 TR 对任何测试水平均无免疫调节作用。日粮添加 TR 被提议作为一种对抗肉鸡中 OA 介导的免疫损伤的方法。
