Penn SRP Center and Center of Excellence in Environmental Toxicology Center, Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Adv Exp Med Biol. 2019;1140:635-647. doi: 10.1007/978-3-030-15950-4_38.
The advancement of mass spectrometry-based analytical platform largely facilitates small-molecule metabolomics studies, which allows simultaneously analysis of a large number of metabolites from bio-samples and give a general picture of metabolic changes related to diseases or environmental alteration. Due to the large diversity of cellular metabolites, globally and precisely examining metabolic profile remains the most challenging part in metabolomic experiment. Mass spectrometry coupled with liquid chromatography enhances sensitivity and resolving power of metabolites identification and quantification, as well as versatility of analyzing a wide array of metabolites. In this chapter, we discussed the technical aspects of each step in the workflow of metabolomics studies we aimed to give technical guidelines for metabolomics investigation design and approach.
基于质谱的分析平台的进步极大地促进了小分子代谢组学研究，它允许同时分析生物样本中大量的代谢物，并给出与疾病或环境变化相关的代谢变化的总体情况。由于细胞代谢物的多样性很大，因此全球范围内准确地检查代谢谱仍然是代谢组学实验中最具挑战性的部分。质谱与液相色谱相结合，提高了代谢物鉴定和定量的灵敏度和分辨率，以及广泛分析各种代谢物的多功能性。在本章中，我们讨论了代谢组学研究工作流程中每个步骤的技术方面，旨在为代谢组学研究设计和方法提供技术指导。
