一种用于在黑腹果蝇中筛选染色体缺失和重复的选择性筛选方法。
A selective screen to recover chromosomal deletions and duplications in Drosophila melanogaster.
作者信息
Gubb D, McGill S, Ashburner M
机构信息
Department of Genetics, University of Cambridge, England.
出版信息
Genetics. 1988 Jun;119(2):377-90. doi: 10.1093/genetics/119.2.377.
Abstract
A screen is described that will select for breakpoints within a restricted chromosomal region in Drosophila. The aberrations recovered can be used to construct chromosomes carrying synthetic duplications and deletions. Such chromosomes have applications in the mapping of complementation groups at both the genetic and molecular level. In particular, breakpoints recovered after P element hybrid dysgenesis tend to be associated with P element insertion sites. Such aberration breakpoints can be genetically mapped, as synthetic deletions, and then used as transposon-tagged sites for the recovery of genomic clones.
摘要
本文描述了一种筛选方法，该方法可在果蝇的一个受限染色体区域内选择断点。回收的畸变可用于构建携带合成重复和缺失的染色体。此类染色体在遗传和分子水平的互补群定位中均有应用。特别地，P 因子杂种不育后回收的断点往往与 P 因子插入位点相关。此类畸变断点可作为合成缺失进行遗传定位，然后用作转座子标记位点以回收基因组克隆。
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本文引用的文献
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Techniques for manipulating chromosomal rearrangements and their application to Drosophila melanogaster. I. Pericentric inversions.染色体重排操作技术及其在黑腹果蝇中的应用。I. 臂间倒位。
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