Baldridge J R, Thomashow M F, Hinrichs D J
Department of Microbiology, Washington State University, Pullman 99164.
Infect Immun. 1988 Aug;56(8):2109-13. doi: 10.1128/iai.56.8.2109-2113.1988.
Events necessary for triggering the cell-mediated response to intracellular parasites are poorly understood. Here we show that extremely high doses of avirulent Listeria monocytogenes 19113 (greater than 10(9] induce a modest and short-lived state of resistance in BALB/c mice. Induction of this protective state could not be achieved with nonviable bacteria and was blocked by inhibiting replication of viable L. monocytogenes 19113 through antibiotic treatment. The immune response was antigen specific and could be adoptively transferred with lymphoid cells. However, unlike the prototypic acquired cellular resistance induced by virulent Listeria strains, the protective response induced by L. monocytogenes 19113 was extremely short-lived, lasting less than 2 weeks, with a precipitous decline in the activity of the immune cells involved. An intervening in vitro culture period with concanavalin A greatly enhanced the activity of the adoptively transferred immune cells.
引发针对细胞内寄生虫的细胞介导反应所必需的事件仍知之甚少。在此我们表明，极高剂量的无毒单核细胞增生李斯特菌19113（大于10⁹）可在BALB/c小鼠中诱导适度且短暂的抗性状态。用无活力的细菌无法实现这种保护状态的诱导，并且通过抗生素处理抑制活的单核细胞增生李斯特菌19113的复制可阻断这种诱导。免疫反应具有抗原特异性，并且可以通过淋巴细胞进行过继转移。然而，与由有毒李斯特菌菌株诱导的典型获得性细胞抗性不同，单核细胞增生李斯特菌19113诱导的保护反应极其短暂，持续时间不到2周，所涉及的免疫细胞活性急剧下降。用伴刀豆球蛋白A进行中间体外培养期可大大增强过继转移的免疫细胞的活性。
