Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, PR China.
Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3281-3286. doi: 10.1099/ijsem.0.003622.
A novel Gram-negative, obligate aerobic, rod-shaped, motile by one or two polar flagella, non-spore-forming bacterial strain, WCHPs060044, was isolated from the wastewater treatment plant at West China Hospital in Chengdu, PR China. Analysis of its 16S rRNA gene sequence showed that strain WCHPs060044 belonged to the genus with the highest similarity to KL28 (99.73 %), HYS (99.52 %) and CCOS 846 (99.38 %). Phylogenomic analysis based on 107 core gene sequences demonstrated that WCHPs060044 was a member of the group but was distant from all closely related species. Whole-genome comparisons, using average nucleotide identity based on blast (ANIb) and DNA-DNA hybridization (isDDH), confirmed low genome relatedness to its close phylogenetic neighbours [below the recommended thresholds of 95 % (ANIb) and 70 % (isDDH) for species delineation]. Phenotypic characterization tests showed that the utilization of l-arginine, l-fucose, d-galacturonic, d-glucuronic acid, inositol and sorbitol in combination could distinguish this strain from other related species of the genus . Therefore, based on genetic and phenotypic evidence, strain WCHPs060044 represents a novel species, for which the name sp. nov. is proposed. The type strain is WCHPs060044 (GDMCC 1.1396=JCM 32907=CCTCC AB 2018120=CNCTC 7663).
一株新的革兰氏阴性、需氧、杆状、通过一个或两个极生鞭毛运动、非孢子形成的细菌菌株，WCHPs060044，从中国成都华西医院的污水处理厂中分离得到。对其 16S rRNA 基因序列的分析表明，菌株 WCHPs060044属于 属，与 KL28（99.73%）、HYS（99.52%）和 CCOS 846（99.38%）的相似度最高。基于 107 个核心基因序列的系统基因组分析表明，WCHPs060044是 群的成员，但与所有密切相关的物种都有一定的距离。全基因组比较，使用基于 blast 的平均核苷酸同一性（ANIb）和 DNA-DNA 杂交（isDDH），证实与密切相关的近缘种的基因组相关性较低[低于物种划分推荐阈值的 95%（ANIb）和 70%（isDDH）]。表型特征测试表明，l-精氨酸、l-岩藻糖、d-半乳糖醛酸、d-葡萄糖醛酸、肌醇和山梨醇的联合利用可以将该菌株与属内的其他相关种区分开来。因此，基于遗传和表型证据，菌株 WCHPs060044代表了一个新的种，建议命名为 sp. nov.。模式菌株为 WCHPs060044（GDMCC 1.1396=JCM 32907=CCTCC AB 2018120=CNCTC 7663）。
