State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China.
College of Life Science, Institute of Life Science and Green Development, Hebei University; Key Laboratory of Microbial Diversity Research and Application of Hebei Province; Engineering Laboratory of Microbial Breeding and Preservation, Baoding, PR China.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6052-6059. doi: 10.1099/ijsem.0.004485.
An aerobic, Gram-stain-negative, rod-shaped bacterium, designated as strain R-22-3 w-18, was isolated from Baiyangdian Lake in Xiong'an New Area, PR China. Growth was observed at 15-37 °C (optimum, 30 °C) and pH 7.0-10.0 (optimum, pH 8.0). 16S rRNA gene sequence analysis placed the strain in a separate branch in the group, with DSM12111 as its closest phylogenetic relative (98.2 %). Whole-genome comparisons, using average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) analyses, confirmed low genome relatedness to the closely related species [below the recommended thresholds of 95 % (ANI), 95 % (AAI) and 70 % (DDH) for species delineation]. Phenotypic characterization tests could also distinguish this strain from other related species of the genus . Therefore, based on genetic and phenotypic evidence, it is clear that strain R-22-3 w-18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R-22-3 w-18 (=CGMCC 1.17250=KCTC 72658).
一株好氧、革兰氏阴性、杆状细菌，被命名为 R-22-3 w-18 菌株，从中国雄安新区白洋淀湖中分离得到。在 15-37°C（最佳温度为 30°C）和 pH 值 7.0-10.0（最佳 pH 值 8.0）条件下观察到生长。16S rRNA 基因序列分析将该菌株置于 组的一个单独分支中，与 DSM12111 为其最接近的系统发育相关物（98.2%）。全基因组比较，使用平均核苷酸同一性（ANI）、平均氨基酸同一性（AAI）和数字 DNA-DNA 杂交（dDDH）分析，确认与密切相关的 种的基因组相关性较低[种划界的推荐阈值以下 95%（ANI）、95%（AAI）和 70%（DDH）]。表型特征测试也可以将该菌株与属内的其他相关种区分开来。因此，基于遗传和表型证据，很明显 R-22-3 w-18 菌株代表了属内的一个新种，建议将其命名为 sp. nov.。模式菌株为 R-22-3 w-18（=CGMCC 1.17250=KCTC 72658）。
