sp. nov.，分离自雄安新区白洋淀。
sp. nov., isolated from Baiyangdian Lake in Xiong'an New Area.
机构信息
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China.
College of Life Science, Institute of Life Science and Green Development, Hebei University; Key Laboratory of Microbial Diversity Research and Application of Hebei Province; Engineering Laboratory of Microbial Breeding and Preservation, Baoding, PR China.
出版信息
Int J Syst Evol Microbiol. 2020 Dec;70(12):6052-6059. doi: 10.1099/ijsem.0.004485.
An aerobic, Gram-stain-negative, rod-shaped bacterium, designated as strain R-22-3 w-18, was isolated from Baiyangdian Lake in Xiong'an New Area, PR China. Growth was observed at 15-37 °C (optimum, 30 °C) and pH 7.0-10.0 (optimum, pH 8.0). 16S rRNA gene sequence analysis placed the strain in a separate branch in the group, with DSM12111 as its closest phylogenetic relative (98.2 %). Whole-genome comparisons, using average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) analyses, confirmed low genome relatedness to the closely related species [below the recommended thresholds of 95 % (ANI), 95 % (AAI) and 70 % (DDH) for species delineation]. Phenotypic characterization tests could also distinguish this strain from other related species of the genus . Therefore, based on genetic and phenotypic evidence, it is clear that strain R-22-3 w-18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R-22-3 w-18 (=CGMCC 1.17250=KCTC 72658).
一株好氧、革兰氏阴性、杆状细菌，被命名为 R-22-3 w-18 菌株，从中国雄安新区白洋淀湖中分离得到。在 15-37°C（最佳温度为 30°C）和 pH 值 7.0-10.0（最佳 pH 值 8.0）条件下观察到生长。16S rRNA 基因序列分析将该菌株置于 组的一个单独分支中，与 DSM12111 为其最接近的系统发育相关物（98.2%）。全基因组比较，使用平均核苷酸同一性（ANI）、平均氨基酸同一性（AAI）和数字 DNA-DNA 杂交（dDDH）分析，确认与密切相关的 种的基因组相关性较低[种划界的推荐阈值以下 95%（ANI）、95%（AAI）和 70%（DDH）]。表型特征测试也可以将该菌株与属内的其他相关种区分开来。因此，基于遗传和表型证据，很明显 R-22-3 w-18 菌株代表了属内的一个新种，建议将其命名为 sp. nov.。模式菌株为 R-22-3 w-18（=CGMCC 1.17250=KCTC 72658）。
Zotero 插件