Qin Jiayuan, Hu Yiyi, Wu Wenjing, Feng Yu, Zong Zhiyong
Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, PR China.
Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, PR China.
Int J Syst Evol Microbiol. 2020 Jul;70(7):4199-4203. doi: 10.1099/ijsem.0.004272.
A novel Gram-negative, obligate aerobic, mobile, rod-shaped and non-spore-forming bacterial strain, WCHP16, was isolated from the wastewater treatment plant at West China Hospital in Chengdu, PR China. It was characterized using a polyphasic approach. Analysis of its 16S rRNA gene sequence showed that strain WCHP16 belonged to the genus with the highest similarity to JJ3 (99.34 %), VM14 (99.0 %), NBRC 103162 (99.0 %) and RYU5 (99.0 %). Phylogenomic analysis based on 107 core gene sequences demonstrated that WCHP16 was a member of the group but was distant from all closely related species. Whole-genome comparisons, using average nucleotide identity based on blast (ANIb) and DNA-DNA hybridization (DDH), confirmed low genome relatedness to all the known species (below the recommended thresholds of 95 % [ANIb] and 70 % [DDH] for species delineation). Phenotypic characterization tests showed that the utilization of phenylacetic acid and capric acid, but not d-arabitol, and inability to produce fluorescent (King B medium) in combination could distinguish this strain from other related species of the genus . Therefore, based on genetic and phenotypic evidence, it is clear that strain WCHP16 represents a novel species, for which the name sp. nov. is proposed. The type strain is WCHP16 (GDMCC1.1215=CCTCC AB 2017103=KCTC 52991).
从中国成都华西医院的污水处理厂分离出一株新型革兰氏阴性、专性需氧、可运动、杆状且无芽孢形成的细菌菌株WCHP16。采用多相分类法对其进行了表征。对其16S rRNA基因序列的分析表明，菌株WCHP16属于该属，与JJ3（99.34%）、VM14（99.0%）、NBRC 103162（99.0%）和RYU5（99.0%）的相似性最高。基于107个核心基因序列的系统基因组分析表明，WCHP16是该组的一个成员，但与所有密切相关的物种距离较远。使用基于blast的平均核苷酸同一性（ANIb）和DNA-DNA杂交（DDH）进行的全基因组比较证实，与所有已知的该物种的基因组相关性较低（低于物种划分推荐的阈值95% [ANIb]和70% [DDH]）。表型特征测试表明，利用苯乙酸和癸酸，但不利用D-阿拉伯糖醇，以及不能在King B培养基中产生荧光，可将该菌株与该属的其他相关物种区分开来。因此，基于遗传和表型证据，很明显菌株WCHP16代表一个新物种，为此提出新物种名称sp. nov.。模式菌株为WCHP16（GDMCC1.1215 = CCTCC AB 2017103 = KCTC 52991）。
