Zhang Yue, Meng Jingjing, Zhang Li, Ramkrishnan Sundaram, Roy Sabita
Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL 33101; and.
Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455.
Immunohorizons. 2019 Jun 4;3(6):186-193. doi: 10.4049/immunohorizons.1900016.
Accumulating evidence shows that extracellular vesicles (EVs) secreted by immune cells play an important role in intercellular communication. In the current report, we show that EVs released from wild-type bone marrow-derived dendritic cells (BMDCs) transfer TLRs to TLR4-knockout (TLR4KO) BMDCs and increase cellular responsiveness to LPS in recipient cells. The transferred EVs have exosomal characteristics and induce the activation of NF-κB signaling pathways in recipient cells. We further show that BMDC-derived EVs can promote LPS-induced inflammation in TLR4KO mice in vivo. These results indicate that functional TLR4 can be transferred from wild-type to TLR4KO BMDCs through exosome-like EVs.
越来越多的证据表明，免疫细胞分泌的细胞外囊泡（EVs）在细胞间通讯中发挥着重要作用。在本报告中，我们发现野生型骨髓来源的树突状细胞（BMDCs）释放的EVs将Toll样受体（TLRs）转移至Toll样受体4基因敲除（TLR4KO）的BMDCs，并增强受体细胞对脂多糖（LPS）的细胞反应性。所转移的EVs具有外泌体特征，并诱导受体细胞中核因子κB（NF-κB）信号通路的激活。我们进一步证明，BMDC来源的EVs可在体内促进TLR4KO小鼠中LPS诱导的炎症反应。这些结果表明，功能性TLR4可通过类外泌体EVs从野生型转移至TLR4KO的BMDCs。
