Fink R, Liberman D F, Murphy K, Lupo D, Israeli E
M.I.T., Environmental Medical Service, Cambridge, MA 02139.
Am Ind Hyg Assoc J. 1988 Jun;49(6):277-9. doi: 10.1080/15298668891379747.
Bacillus subtilis var. niger spores were used to determine the exposure time for formaldehyde decontamination of biological safety cabinets. Formaldehyde contact times less than 3 hr were insufficient for sterilization. A contact time of 4 hr or more resulted in a reproducible killing of the spore strips placed inside the cabinets. At 6 hr sufficient formaldehyde had diffused through the high efficiency particulate air (HEPA) filter to sterilize the strips with lower spore counts. A minimum of 5 to 6 logs of killing were observed after 4 to 6 hr of treatment.
使用枯草芽孢杆菌黑色变种孢子来确定生物安全柜甲醛消毒的暴露时间。甲醛接触时间少于3小时不足以灭菌。4小时或更长时间的接触时间可使放置在柜内的孢子条实现可重复的杀灭。6小时时，足够的甲醛已扩散通过高效空气过滤器（HEPA），从而对孢子数较低的条带进行灭菌。处理4至6小时后观察到至少5至6个对数的杀灭效果。
