Three Novel Monoterpenoid Glycosides From Fruits Of .

The phytochemical investigation on the fruits of (Araliaceae) resulted in the discovery of three novel monoterpene glycosides, eleuhenryiside A (), eleuhenryiside B (), and eleuhenryiside C (), as well as a known lignan, (-)-kobusin (). Their chemical structures were elucidated by mass, 1 D- and 2 D-NMR spectroscopy. The chemical structures of new compounds were determined to be (2,6)-6-hydroxy-2,6-dimethyl-2,7-octadien-1-yl-(6'--acetyl)---glucopyranoside, (2,6)-6-hydroxy-2,6-dimethyl-2,7-octadien-1-yl-(6'--acetyl)---glucopyranoside, and (-)-(4 )-4,7-dihydroxy-1-menthene 7---glucopyranoside, respectively. The anti-neuroinflammatory and anti-inflammatory activities of these compounds were evaluated with LPS-stimulated BV2 microglia and RAW264.7 macrophage, respectively. The results showed that new compounds and have inhibitory effects of NO production with IC values of 32.50 ± 1.60 and 3.54 ± 0.20 μM in LPS-stimulated BV2 microglia. Also, (-)-kobusin () has abilities to inhibit NO production with the IC values of 14.25 ± 2.69 and 36.35 ± 6.27 μM in BV2 and RAW264.7 cells, respectively, which indicated that it may possess the potential anti-neuroinflammatory and anti-inflammatory activities.