Elowni Elsayed E, Ahmad Mohamed Fadl, Abdelnabi Ghada H, Badawi Reem M
Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, Shambat, Sudan.
Open Vet J. 2019 Jul;9(2):172-176. doi: 10.4314/ovj.v9i2.13. Epub 2019 Jun 22.
Methylene blue was used as a vital stain for the assessment of viability of protoscolices from hydatid cysts taking advantage of the chemical nature of the dye as a redox indicator and the kinetically distinct molecular transfer systems of protoscolex for uptake of materials across the tegument.
The present study attempts to validate the application of methylene blue staining for assessment of viability of protoscolices.
To validate the criteria by which viability is assessed, control tests were performed using normal protoscolices and protoscolices previously treated with distilled water at 60°C for 5 minutes. Performance of methylene blue was further studied at intervals over a period of 50 minutes after protoscolex exposure using 1% dye concentration.
Normal protoscolices were able to adsorb and reduce the dye and have, therefore, lost the blue color. Protoscolices previously treated with warm water on the other hand, being functionally dead, failed to reduce the adsorbed dye and permanently retained the blue color. Results also indicated that a clear distinction between dead and alive protoscolices can be made within 1 minute. Reading of the test after 10 minutes would be misleading giving a false result.
These findings suggest that viability of protoscolices can be assessed on the basis of acquisition and loss/retaining of the dye blue color. Increasing the concentration of methylene blue to 1% was noticed to be associated with remarkable enhancement of contractility, sucker movement, and evagination. Such an excitatory action of the dye may be exploited in viability tests which adopt these criteria.
利用亚甲蓝作为氧化还原指示剂的化学性质以及原头节独特的分子转运系统来摄取穿过皮层的物质，将其用作活体染色剂以评估包虫囊肿中原头节的活力。
本研究旨在验证亚甲蓝染色在评估原头节活力方面的应用。
为验证评估活力的标准，使用正常原头节和先前在60°C蒸馏水中处理5分钟的原头节进行对照试验。在原头节暴露后50分钟内，每隔一段时间使用1%的染料浓度进一步研究亚甲蓝的性能。
正常原头节能够吸附并还原染料，因此失去了蓝色。另一方面，先前用温水处理过的原头节，由于功能上已死亡，无法还原吸附的染料并永久保留蓝色。结果还表明，在1分钟内可以明确区分死亡和存活的原头节。10分钟后读取测试结果会产生误导，得出错误结果。
这些发现表明，可以根据染料蓝色的摄取和丧失/保留情况来评估原头节的活力。注意到将亚甲蓝浓度提高到1%与收缩性、吸盘运动和外翻的显著增强有关。染料的这种兴奋作用可用于采用这些标准的活力测试中。
