Ebrahimi Zohreh, Khazaei Mohammad Rasool, Ghanbari Elham, Khazaei Mozafar
Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Adv Biomed Res. 2019 Jun 28;8:41. doi: 10.4103/abr.abr_65_19. eCollection 2019.
Diabetic nephropathy is a complex and multifactorial adverse effect of diabetes mellitus (DM). Crab shell as a natural product is supposed to have antioxidant effect which is one of the important mechanisms to improve DM. The aim of this study was to investigate the effect of crab shell extract (CSE) on the histopathology and antioxidant status of kidney in diabetic rats.
Forty-two adult Wistar rats (210 ± 10 g) were divided into six groups ( = 7). Streptozotocin (50 mg/kg) was administered interaperitonealy (IP) for inducing diabetes. Rats were treated for 14 days by CSE with 100, 200, and 400 mg/kg doses IP. Fasting blood glucose, body, and renal weight were evaluated. The antioxidant status of kidney's tissue was evaluated by determining the level of ferric-reducing antioxidant power (FRAP). Furthermore, urine samples were used to determine nitric oxide (NO) levels. Microscopic slides were prepared to compare kidney histology between groups. Data were analyzed by one-way analysis of variance with Tukey's test, and < 0.05 was considered statistically significant.
CSE induced a significant reduction in blood glucose ( = 0.01) and a significant increase in total antioxidant capacity (FRAP) ( = 0.004). Furthermore, urine NO was decreased significantly ( = 0.000). The extract improved renal tissue changes caused by diabetes.
CSE improved antioxidant status and diabetic histological changes of rat kidney, and it could be an alternative complementary therapy in diabetic-associated disorders.
糖尿病肾病是糖尿病（DM）一种复杂的多因素不良反应。蟹壳作为一种天然产物，被认为具有抗氧化作用，这是改善糖尿病的重要机制之一。本研究的目的是探讨蟹壳提取物（CSE）对糖尿病大鼠肾脏组织病理学及抗氧化状态的影响。
将42只成年Wistar大鼠（210±10 g）分为6组（每组n = 7）。腹腔注射链脲佐菌素（50 mg/kg）诱导糖尿病。大鼠腹腔注射100、200和400 mg/kg剂量的CSE，治疗14天。评估空腹血糖、体重和肾脏重量。通过测定铁还原抗氧化能力（FRAP）来评估肾脏组织的抗氧化状态。此外，用尿液样本测定一氧化氮（NO）水平。制备显微玻片以比较各组之间的肾脏组织学。数据采用单因素方差分析和Tukey检验进行分析，P < 0.05被认为具有统计学意义。
CSE使血糖显著降低（P = 0.01），总抗氧化能力（FRAP）显著增加（P = 0.004）。此外，尿NO显著降低（P = 0.000）。该提取物改善了糖尿病引起的肾脏组织变化。
CSE改善了大鼠肾脏的抗氧化状态和糖尿病组织学变化，可能是糖尿病相关疾病的一种替代补充疗法。
