Kenya Medical Research Institute, Nairobi, Kenya.
College of Health Sciences (COHES), Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
Biomed Res Int. 2019 Jul 7;2019:4798906. doi: 10.1155/2019/4798906. eCollection 2019.
Cystic Echinococcosis (CE) is a widespread neglected zoonotic disease and is caused by the larval stage of the dog tapeworm sensu lato. CE is more frequent in livestock-rearing areas and where people live a nomadic or seminomadic lifestyle such as in Kajiado County, Kenya. There is limited data on CE disease situation in the county of Maasailand; the present study, therefore, reports on the prevalence of CE in cattle, sheep, and goats and their relative importance in CE transmission in Kajiado County. In total, 1,486 livestock (388 cattle, 625 sheep, and 473 goats) slaughtered in two abattoirs were examined for the presence of hydatid cysts in various organs. Cyst isolates were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the NADH dehydrogenase subunit 1 gene (). The overall prevalence of CE was 14.8% (220/1486), while prevalence per livestock species was 15.2% (72/473) in goats, 14.9% (93/625) in sheep, and 14.2% (55/388) in cattle. Out of the 421 cysts isolated, 389 cysts were successfully characterized to be either sensu stricto (s. s.), 356/389 (91.5%), (G6/7), 26/389 (6.7%), or , 7/389 (1.8%). This record confirms predominance of s. s. in Maasailand and other parts of Kenya, while the importance of and (G6/7) to the general CE burden in Maasailand might be higher than previously thought. More so, a higher infection pressure for humans by s. s. based on its abundance could be speculated. The study sheds significant light on CE situation in livestock in the nomadic/seminomadic society of the Maasai in Kajiado County and provides good bases to investigate human CE in the area.
泡型包虫病（CE）是一种广泛存在的被忽视的人畜共患疾病，由犬带绦虫的幼虫阶段引起。CE 在畜牧业地区以及肯尼亚卡贾多县等游牧或半游牧生活方式的人群中更为常见。关于马赛人地区的 CE 疾病情况，数据有限；因此，本研究报告了卡贾多县牛、羊和山羊中 CE 的流行情况及其在 CE 传播中的相对重要性。在两个屠宰场共检查了 1486 头牲畜（388 头牛、625 只羊和 473 只山羊），以检测各种器官中是否存在包虫囊肿。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对烟酰胺脱氢酶亚单位 1 基因（）的基因分型对囊肿分离株进行了鉴定。CE 的总流行率为 14.8%（220/1486），而每种牲畜的流行率为山羊 15.2%（72/473）、绵羊 14.9%（93/625）和牛 14.2%（55/388）。在分离出的 421 个囊肿中，成功鉴定了 389 个囊肿为 sensu stricto（s. s.）、356/389（91.5%）、 （G6/7）、26/389（6.7%）或 ，7/389（1.8%）。这一记录证实了 s. s. 在马赛兰和肯尼亚其他地区的流行，而 和 （G6/7）对马赛兰一般 CE 负担的重要性可能比以前认为的要高。此外，基于其丰富度，s. s. 可能会对人类造成更高的感染压力。该研究为卡贾多县马赛游牧/半游牧社会的牲畜中的 CE 情况提供了重要信息，并为该地区的人类 CE 调查提供了良好的基础。
