Department of Gynecology and Obstetrics, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Key Laboratory of Gynecologic Oncology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
BMC Infect Dis. 2019 Aug 1;19(1):677. doi: 10.1186/s12879-019-4279-6.
In this study, the association between high-risk human papillomavirus (hrHPV) infection and the vaginal microbiome in pregnant women was evaluated in Chinese cohorts.
The vaginal bacterial composition of four groups, 38 hrHPV-infected pregnant women (PHR, n = 38), pregnant women without HPV infection (PN, n = 48), nonpregnant women with hrHPV infection (NPHR, n = 19) and nonpregnant women without HPV infection (NPN, n = 30), was characterized by deep sequencing of barcoded 16S rRNA gene fragments (V3-4) using Illumina MiSeq.
The results revealed that both pregnancy and HPV infection can increase vaginal bacterial microbial richness and diversity, with the bacterial composition being most influenced by pregnancy. Lactobacillus was the most dominant genus among all samples. NPN samples were dominated by CST (community state type) III, mainly composed of Lactobacillus iners. Both pregnancy and hrHPV infection were accompanied by an increased proportion of CST I (dominated by Lactobacillus crispatus), as opposed to CST III. Bifidobacterium, Bacillus, Megasphaera, Sneathia, Prevotella, Gardnerella, Fastidiosipila and Dialister were found to be biomarkers for hrHPV-infected women, though different genera (Bifidobacterium, Megasphaera, Bacillus, Acidovorax, Oceanobacillus and Lactococcus) were associated with hrHPV-infected pregnant women.
This work uncovered a probable synergistic effect of hrHPV infection and pregnancy on the vaginal microbial composition. HPV infection in pregnant women was associated with a more complex and diverse microbial environment.
本研究评估了中国人群中高危型人乳头瘤病毒（hrHPV）感染与孕妇阴道微生物组之间的关联。
采用 Illumina MiSeq 对 4 组人群（38 例 hrHPV 感染孕妇[PHR]，n=38；HPV 未感染孕妇[PN]，n=48；HPV 未感染非妊娠妇女[NPHR]，n=19；HPV 未感染非妊娠妇女[NPN]，n=30）的阴道细菌组成进行了深度测序，测序片段为 16S rRNA 基因的条形码 V3-4 区。
结果表明，妊娠和 HPV 感染均可增加阴道细菌微生物丰富度和多样性，而妊娠对细菌组成的影响最大。所有样本中乳杆菌属均为最主要的菌属。NPN 样本以 CST（群落状态类型）III 为主，主要由惰性乳杆菌组成。妊娠和 hrHPV 感染均伴随着 CST I（以卷曲乳杆菌为主）比例的增加，而非 CST III。双歧杆菌属、芽孢杆菌属、巨球形菌属、西氏菌属、普雷沃氏菌属、加德纳菌属、迟缓真杆菌属和消化链球菌属被认为是 hrHPV 感染女性的生物标志物，但不同的属（双歧杆菌属、巨球形菌属、芽孢杆菌属、食酸菌属、海洋杆菌属和乳球菌属）与 hrHPV 感染的孕妇有关。
本研究揭示了 hrHPV 感染和妊娠对阴道微生物组成可能存在协同作用。孕妇 HPV 感染与更复杂、多样化的微生物环境有关。
