Dehghan Farnaz, Boozarpour Sohrab, Torabizadeh Zhila, Alijanpour Sakineh
Department of Biology, Faculty of Basic Sciences, Gonbad kavous University, Gonbad kavous, Golestan, Iran.
Department of Medical Pathology, Faculty of Medicine, Sari University of Medical Sciences, Sari, Mazandaran, Iran.
Onco Targets Ther. 2019 Jul 11;12:5601-5607. doi: 10.2147/OTT.S199508. eCollection 2019.
The aim of this study was to compare the expression of gene in stages II-IV of formalin-fixed paraffin-embedded (FFPE) tissue in patients with colon cancer and introduce as a potential molecular marker for detection of colon cancer in the early stages.
Currently, identification of key molecules involved in the pathogenesis of cancer is one of the areas under consideration. miRNAs, are small RNAs which have been identified in many cancers. In this study, we investigated the expression of in three pathologic stages in patients with colon cancer in the north of Iran.
A total of 40 FFPE samples were obtained from patients with stages II, III, and IV from hospitals in Mazandaran and Golestan provinces. After extraction of RNA, treatment with DNase I and cDNA synthesis was performed and expression was assessed by qPCR. Then, the data were analyzed using statistical software R (3.4.3).
The expression of in stage II was significantly different from stage IV. However, no significant difference was observed between the other stages. In stage II, the level of expression was higher in men than women. Moreover, in the second pathological stage, miR-21 expression was reduced in patients with adjacent lymphoid tissue engagement. In addition, the expression of in grade I was significantly higher than grade II.
The results of this study suggest that can be a diagnostic marker for early stages of colon cancer, especially in men. It can also be considered as a good candidate for targeted treatment of colon cancer in the early stages of the disease. Furthermore, for the first time, we suggested that can be a good molecular marker for classification of the stages of colon cancer.
本研究旨在比较结肠癌患者福尔马林固定石蜡包埋（FFPE）组织II - IV期基因的表达情况，并引入该基因作为早期检测结肠癌的潜在分子标志物。
目前，确定参与癌症发病机制的关键分子是正在研究的领域之一。微小RNA（miRNAs）是在许多癌症中已被鉴定出的小RNA。在本研究中，我们调查了伊朗北部结肠癌患者三个病理阶段的该基因表达情况。
从马赞德兰省和戈勒斯坦省的医院获取了共40份II、III和IV期患者的FFPE样本。提取RNA后，进行DNA酶I处理和cDNA合成，并通过qPCR评估该基因表达。然后，使用统计软件R（3.4.3）对数据进行分析。
该基因在II期和IV期的表达存在显著差异。然而，其他阶段之间未观察到显著差异。在II期，男性的该基因表达水平高于女性。此外，在第二个病理阶段，伴有相邻淋巴组织受累的患者中miR - 21表达降低。另外，I级中的该基因表达明显高于II级。
本研究结果表明，该基因可作为结肠癌早期的诊断标志物，尤其是在男性中。它也可被视为疾病早期结肠癌靶向治疗的良好候选物。此外，我们首次提出该基因可作为结肠癌分期分类的良好分子标志物。
