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种:细菌吲哚-3-乙酸的高效微型工厂。

spp.: potent microfactories of bacterial IAA.

作者信息

Wagi Shabana, Ahmed Ambreen

机构信息

Department of Botany, University of the Punjab, Lahore, Punjab, Pakistan.

出版信息

PeerJ. 2019 Jul 23;7:e7258. doi: 10.7717/peerj.7258. eCollection 2019.

Abstract

BACKGROUND

Auxin production by bacteria is one of the most important direct mechanisms utilized by plant growth-promoting bacteria (PGPB) for the betterment of plants naturally because auxin is a plant friendly secondary metabolite synthesized naturally by bacteria, and hence improves the growth of associated plants. So, the current study focuses on bacterial synthesis of Indole-3-acetic acid (IAA) for plant growth improvement.

METHODS

In the current study, the PGPB were selected on the basis of their auxin production potential and their growth promoting attributes were evaluated. Indole-3-acetic acid producing potential of two selected bacterial isolates was observed by varying different growth conditions i.e., media composition, carbon sources (glucose, sucrose and lactose) and different concentrations of precursor. Influence of various physiological factors (temperature and incubation time period) on IAA production potential was also evaluated.

RESULTS

Both the bacterial strains (So3II) and (Mt3b) showed variable potential for the production of bacterial IAA under different set of growth and environmental conditions. Hence, the IAA production potential of the bacterial isolates can be enhanced by affecting optimum growth conditions for bacterial isolates and can be used for the optimal production of bacterial IAA and its utilization for plant growth improvement can lead to better yield in an eco-friendly manner.

摘要

背景

细菌产生生长素是植物促生细菌(PGPB)自然改善植物生长所利用的最重要直接机制之一,因为生长素是细菌自然合成的对植物有益的次生代谢产物,从而促进相关植物的生长。因此,本研究聚焦于细菌合成吲哚 - 3 - 乙酸(IAA)以促进植物生长。

方法

在本研究中,根据PGPB产生生长素的潜力选择菌株,并评估其促生长特性。通过改变不同生长条件,即培养基成分、碳源(葡萄糖、蔗糖和乳糖)以及不同浓度的前体,观察两种选定细菌分离株产生吲哚 - 3 - 乙酸的潜力。还评估了各种生理因素(温度和培养时间段)对IAA产生潜力的影响。

结果

两株细菌菌株(So3II)和(Mt3b)在不同的生长和环境条件下表现出不同的产生细菌IAA的潜力。因此,通过影响细菌分离株的最佳生长条件,可以提高细菌分离株的IAA产生潜力,并且可用于细菌IAA的最佳生产,其用于改善植物生长能够以生态友好的方式带来更好的产量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e34e/6659656/d895c16a9e61/peerj-07-7258-g001.jpg

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