University of Tennessee Health Science Center, Memphis, TN, USA.
Sci Rep. 2019 Aug 2;9(1):11270. doi: 10.1038/s41598-019-47703-6.
Septic shock is a devastating health condition caused by uncontrolled sepsis. Advancements in high-throughput sequencing techniques have increased the number of potential genetic biomarkers under review. Multiple genetic markers and functional pathways play a part in development and progression of pediatric septic shock. We identified 53 differentially expressed pediatric septic shock biomarkers using gene expression data sampled from 181 patients admitted to the pediatric intensive care unit within the first 24 hours of their admission. The gene expression signatures showed discriminatory power between pediatric septic shock survivors and nonsurvivor types. Using functional enrichment analysis of differentially expressed genes, we validated the known genes and pathways in septic shock and identified the unexplored septic shock-related genes and functional groups. Differential gene expression analysis revealed the genes involved in the immune response, chemokine-mediated signaling, neutrophil chemotaxis, and chemokine activity and distinguished the septic shock survivor from non-survivor. The identification of the septic shock gene biomarkers may facilitate in septic shock diagnosis, treatment, and prognosis.
脓毒症休克是一种由失控性脓毒症引起的破坏性健康状况。高通量测序技术的进步增加了正在审查的潜在遗传生物标志物的数量。多个遗传标志物和功能途径参与了儿科脓毒性休克的发展和进展。我们使用从 181 名在入住儿科重症监护病房后 24 小时内入院的患者中采集的基因表达数据，鉴定出 53 个差异表达的儿科脓毒性休克生物标志物。基因表达特征显示了儿科脓毒性休克幸存者和非幸存者类型之间的区分能力。通过对差异表达基因的功能富集分析，我们验证了脓毒性休克中已知的基因和途径，并确定了未探索的与脓毒性休克相关的基因和功能组。差异基因表达分析揭示了参与免疫反应、趋化因子介导的信号转导、中性粒细胞趋化性和趋化因子活性的基因，并将脓毒性休克幸存者与非幸存者区分开来。脓毒性休克基因生物标志物的鉴定可能有助于脓毒性休克的诊断、治疗和预后。
