Savino Francesco, Galliano Ilaria, Savino Andrea, Daprà Valentina, Montanari Paola, Calvi Cristina, Bergallo Massimiliano
Department of Paediatrics, Azienda Ospedaliera Universitaria Città della Salute e della Scienza di Torino, Turin, Italy.
Department of Public Health and Paediatric Sciences, Scuola di Medicina, Università degli Studi di Torino, Turin, Italy.
Front Pediatr. 2019 Jul 16;7:292. doi: 10.3389/fped.2019.00292. eCollection 2019.
Studies have shown that ri probiotics can affect cells that play a key role in the immune system. This Italian study investigated how DSM 17938 influenced CC-chemokine receptor 7 (CCR7) and interleukin 10 (IL-10) in breastfed colicky infants. Our University hospital in Turin recruited 50 healthy outpatients, at a median age of approximately 1 month, from September 2017 to August 2018. They were randomized to daily DSM17938 (1 × 10 cfu) or a placebo for 28 days from recruitment. We collected peripheral blood and evaluated the expression of CCR7 messenger ribonucleic acid using the real-time TaqMan reverse transcription polymerase chain reaction method at baseline and after the study period. We found increased expression of CC-chemokine receptor 7 in infants treated with the probiotic, but not the controls ( < 0.0026). No differences were observed for interleukin 10 after the study period in either group. At baseline, daily crying time was comparable in the probiotic and control groups: 341 (25) vs. 337 (29) min., respectively ( = 0.450). After 28 days, daily mean crying time decrease statistically in the probiotic group: 78 (23) vs. 232 (31), respectively ( < 0.001). The increase in CC-chemokine receptor 7 might have been a response to probiotic treatment. As a relatively small sample was used to conduct this study, our research needs to be replicated in different settings, and over time, to produce comparable findings.
研究表明，某些益生菌可影响在免疫系统中起关键作用的细胞。这项意大利研究调查了DSM 17938对母乳喂养的腹绞痛婴儿体内C-C趋化因子受体7（CCR7）和白细胞介素10（IL-10）的影响。我们位于都灵的大学医院在2017年9月至2018年8月期间招募了50名健康门诊患者，中位年龄约为1个月。从招募开始，他们被随机分为每日服用DSM17938（1×10 cfu）或安慰剂组，为期28天。我们采集外周血，并在基线和研究期结束后使用实时TaqMan逆转录聚合酶链反应方法评估CCR7信使核糖核酸的表达。我们发现，服用益生菌的婴儿体内C-C趋化因子受体7的表达增加，而对照组则没有（P<0.0026）。研究期结束后，两组白细胞介素10均未观察到差异。基线时，益生菌组和对照组的每日哭闹时间相当：分别为341（25）分钟和337（29）分钟（P = 0.450）。28天后，益生菌组的每日平均哭闹时间有统计学意义的下降：分别为78（23）分钟和232（31）分钟（P<0.001）。C-C趋化因子受体7的增加可能是对益生菌治疗的反应。由于本研究使用的样本相对较小，我们的研究需要在不同环境中进行重复，并随着时间推移得出可比的结果。
