Zeng Zhen, Yang Yichen, Wu Hengyu
Queen Mary University of London, Nanchang University Nanchang 330006, China.
Department of Breast Surgery, The Second Affiliated Hospital of Nanchang University Nanchang 330006, China.
Am J Transl Res. 2019 Jul 15;11(7):4500-4507. eCollection 2019.
To detect the expression pattern of microRNA-765 in breast cancer (BCa) and its regulatory effect on the disease progression. Expression level of microRNA-765 in 66 paired BCa tissues and matched normal tissues was detected by qRT-PCR. The relationship between microRNA-765 level and clinical data of BCa patients was analyzed. Subsequently, microRNA-765 level in BCa cell lines was examined as well. Changes in proliferative, migratory and invasive abilities in MCF-7 and SKBR3 cells either overexpressing microRNA-765 or not were evaluated. Furthermore, expression level of EZH1 in BCa tissues and cell lines was determined. The regulatory interaction between microRNA-765 and EZH1 was identified. Finally, the role of microRNA-765/EZH1 axis in the progression of BCa was assessed. MicroRNA-765 was downregulated in BCa tissues relative to matched normal ones. BCa patients expressing low expression of microRNA-765 presented higher tumor stage, higher metastatic rate and worse overall survival. Overexpression of microRNA-765 attenuated proliferative, migratory and invasive abilities in MCF-7 and SKBR3 cells. In addition, EZH1 was upregulated in BCa tissues and cell lines. EZH1 level was negatively regulated by microRNA-765 in BCa. Overexpression of EZH1 reversed the inhibitory effects of microRNA-765 on malignant progression of BCa. MicroRNA-765 is downregulated in BCa and closely correlated to tumor stage, lymphatic metastasis, distant metastasis and poor prognosis of BCa patients. Overexpression of microRNA-765 attenuates the malignant progression of BCa through negatively regulating EZH1.
检测微小RNA-765在乳腺癌（BCa）中的表达模式及其对疾病进展的调控作用。采用qRT-PCR检测66对BCa组织及其配对正常组织中微小RNA-765的表达水平。分析微小RNA-765水平与BCa患者临床资料的关系。随后，检测BCa细胞系中微小RNA-765的水平。评估过表达或未过表达微小RNA-765的MCF-7和SKBR3细胞增殖、迁移和侵袭能力的变化。此外，测定BCa组织和细胞系中EZH1的表达水平。确定微小RNA-765与EZH1之间的调控相互作用。最后，评估微小RNA-765/EZH1轴在BCa进展中的作用。与配对的正常组织相比，BCa组织中微小RNA-765表达下调。微小RNA-765低表达的BCa患者具有更高的肿瘤分期、更高的转移率和更差的总生存期。过表达微小RNA-765可减弱MCF-7和SKBR3细胞的增殖、迁移和侵袭能力。此外，BCa组织和细胞系中EZH1表达上调。在BCa中，EZH1水平受到微小RNA-765的负调控。过表达EZH1可逆转微小RNA-765对BCa恶性进展的抑制作用。微小RNA-765在BCa中表达下调，且与BCa患者的肿瘤分期、淋巴转移、远处转移及预后不良密切相关。过表达微小RNA-765通过负调控EZH1减弱BCa的恶性进展。
