Chen Shoukun, Zhao Hongyan, Luo Tengli, Liu Yue, Nie Xiaojun, Li Haifeng
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712000, China.
Plants (Basel). 2019 Aug 8;8(8):274. doi: 10.3390/plants8080274.
Myelocytomatosis oncogenes (MYC) transcription factors (TFs) belong to basic helix-loop-helix (bHLH) TF family and have a special bHLH_MYC_N domain in the N-terminal region. Presently, there is no detailed and systematic analysis of MYC TFs in wheat, rice, and . In this study, 26 TaMYC, 7 OsMYC, and 7 BdMYC TFs were identified and their features were characterized. Firstly, they contain a JAZ interaction domain (JID) and a putative transcriptional activation domain (TAD) in the bHLH_MYC_N region and a BhlH region in the C-terminal region. In some cases, the bHLH region is followed by a leucine zipper region; secondly, they display tissue-specific expression patterns: wheat genes are mainly expressed in leaves, rice genes are highly expressed in stems, and genes are mainly expressed in inflorescences. In addition, three types of -elements, including plant development/growth-related, hormone-related, and abiotic stresses-related were identified in different gene promoters. In combination with the previous studies, these results indicate that MYC TFs mainly function in growth and development, as well as in response to stresses. This study laid a foundation for the further functional elucidation of genes.
髓细胞瘤致癌基因（MYC）转录因子（TFs）属于碱性螺旋-环-螺旋（bHLH）转录因子家族，在N端区域有一个特殊的bHLH_MYC_N结构域。目前，尚未对小麦、水稻和……中的MYC转录因子进行详细而系统的分析。在本研究中，鉴定了26个TaMYC、7个OsMYC和7个BdMYC转录因子，并对其特征进行了表征。首先，它们在bHLH_MYC_N区域包含一个JAZ相互作用结构域（JID）和一个推定的转录激活结构域（TAD），在C端区域有一个BhlH结构域。在某些情况下，BhlH结构域后面跟着一个亮氨酸拉链结构域；其次，它们表现出组织特异性表达模式：小麦基因主要在叶片中表达，水稻基因在茎中高表达，而……基因主要在花序中表达。此外，在不同的……基因启动子中鉴定出三种类型的……元件，包括与植物发育/生长相关、与激素相关和与非生物胁迫相关的元件。结合先前的研究，这些结果表明MYC转录因子主要在生长发育以及对胁迫的响应中发挥作用。本研究为进一步阐明……基因的功能奠定了基础。
