Cao Shijiang, Cheng Han, Zhang Jiashuo, Aslam Mohammad, Yan Maokai, Hu Anqi, Lin Lili, Ojolo Simon Peter, Zhao Heming, Priyadarshani S V G N, Yu Yuan, Cao Guangqiu, Qin Yuan
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China.
Chinese Fir Engineering Technology Research Center under National Forestry and Grassland Administration, Fuzhou 350002, Fujian Province, China.
Plants (Basel). 2019 Aug 8;8(8):275. doi: 10.3390/plants8080275.
The cellulose synthase (Ces) and cellulose synthase-like (Csl) gene families belonging to the cellulose synthase gene superfamily, are responsible for the biosynthesis of cellulose and hemicellulose of the plant cell wall, and play critical roles in plant development, growth and evolution. However, the gene family remains to be characterized in pineapple, a highly valued and delicious tropical fruit. Here, we carried out genome-wide study and identified a total of seven genes and 25 genes in pineapple. Genomic features and phylogeny analysis of genes were carried out, including phylogenetic tree, chromosomal locations, gene structures, and conserved motifs identification. In addition, we identified 32 pineapple genes with 31 genes as orthologs by the syntenic and phylogenetic approaches. Furthermore, a RNA-seq investigation exhibited the expression profile of several genes in various tissues and multiple developmental stages. Collectively, we provided comprehensive information of the evolution and function of pineapple gene superfamily, which would be useful for screening out and characterization of the putative genes responsible for tissue development in pineapple. The present study laid the foundation for future functional characterization of genes in pineapple.
属于纤维素合酶基因超家族的纤维素合酶（Ces）和类纤维素合酶（Csl）基因家族，负责植物细胞壁纤维素和半纤维素的生物合成，并在植物发育、生长和进化中发挥关键作用。然而，在备受珍视且美味的热带水果菠萝中，该基因家族仍有待鉴定。在此，我们进行了全基因组研究，在菠萝中总共鉴定出7个Ces基因和25个Csl基因。对Ces基因进行了基因组特征和系统发育分析，包括系统发育树、染色体定位、基因结构和保守基序鉴定。此外，通过共线性和系统发育方法，我们鉴定出32个菠萝Csl基因，其中31个基因作为直系同源基因。此外，一项RNA测序研究展示了多个Csl基因在不同组织和多个发育阶段的表达谱。总体而言，我们提供了菠萝Csl基因超家族进化和功能的全面信息，这将有助于筛选和鉴定负责菠萝组织发育的假定基因。本研究为未来菠萝Csl基因的功能鉴定奠定了基础。
