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从头组装并发现参与对……反应的基因。 (你提供的原文中“to”后面缺少具体内容,翻译可能不太完整准确)

De novo assembly and discovery of genes involved in the response of to .

作者信息

Wu Liyan, Du Guanghui, Bao Rui, Li Zhibin, Gong Yaju, Liu Feihu

机构信息

1Plant Improvement and Utilization Lab, Yunnan University, Kunming, 650091 Yunnan China.

2Horticultural Institute of Yunnan Academy of Agricultural Sciences, Kunming, 650205 Yunnan China.

出版信息

Physiol Mol Biol Plants. 2019 Jul;25(4):1009-1027. doi: 10.1007/s12298-019-00666-4. Epub 2019 May 13.

Abstract

Verticillium wilt, caused by the soil-borne fungus , is a devastating disease of eggplant ( spp.) and causes substantial losses worldwide. Although some genes or biological processes involved in the interaction between eggplant and have been identified in some studies, the underlying molecular mechanism is not yet clear. Here, we monitored the transcriptomic profiles of the roots of resistant plants challenged with . Based on the measurements of physiological indexes (T-SOD, POD and SSs), three time points were selected and subsequently divided into two stages (S_12 h vs. S_0 h and S_48 h vs. S_12 h). KEGG enrichment analysis of the DEGs revealed several genes putatively involved in regulating plant- interactions, including mitogen-activated protein kinase (MAPK) genes (MEKK1 and MAP2K1), WRKY genes (WRKY22 and WRKY33) and cytochrome P450 (CYP) genes (CYP73A/C4H, CYP98A/C3'H and CYP84A/F5H). In addition, a subset of genes that play an important role in activating defence responses, including genes as well as genes encoding PR proteins and TFs, were screened and are discussed. These results will help to identify key resistance genes and will contribute to a further understanding of molecular mechanisms of the resistance response to

摘要

黄萎病由土壤传播的真菌引起,是茄子的一种毁灭性病害,在全球范围内造成重大损失。尽管一些研究已经鉴定出茄子与该真菌相互作用中涉及的一些基因或生物学过程,但其潜在的分子机制尚不清楚。在此,我们监测了用该真菌攻击的抗性茄子植株根系的转录组图谱。基于生理指标(T-SOD、POD和SSs)的测定,选择了三个时间点,随后分为两个阶段(S_12小时与S_0小时以及S_48小时与S_12小时)。对差异表达基因(DEGs)的KEGG富集分析揭示了几个可能参与调节植物与该真菌相互作用的基因,包括丝裂原活化蛋白激酶(MAPK)基因(MEKK1和MAP2K1)、WRKY基因(WRKY22和WRKY33)以及细胞色素P450(CYP)基因(CYP73A/C4H、CYP98A/C3'H和CYP84A/F5H)。此外,筛选并讨论了一组在激活茄子防御反应中起重要作用的基因,包括该真菌基因以及编码病程相关蛋白(PR)和转录因子(TFs)的基因。这些结果将有助于鉴定关键抗性基因,并有助于进一步了解茄子对该真菌抗性反应的分子机制。

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