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利用NZ9000生物合成多糖包覆的硒纳米颗粒及其抗氧化和抗炎活性

Biosynthesis of Polysaccharides-Capped Selenium Nanoparticles Using NZ9000 and Their Antioxidant and Anti-inflammatory Activities.

作者信息

Xu Chunlan, Qiao Lei, Ma Li, Yan Shuqi, Guo Yu, Dou Xina, Zhang Baohua, Roman Alexandra

机构信息

The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, China.

出版信息

Front Microbiol. 2019 Jul 26;10:1632. doi: 10.3389/fmicb.2019.01632. eCollection 2019.

DOI:10.3389/fmicb.2019.01632
PMID:31402902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6676592/
Abstract

() NZ9000, which has been genetically modified, is the most commonly used host strain for nisin regulated gene expression. Selenium (Se) is an essential trace element in the diet of humans and animals important for the maintenance of health and growth. Biosynthesized Se nanoparticles (SeNPs) that use microorganisms as a vehicle are uniquely advantages in terms of low costs, low toxicity and high bioavailability. This study was aimed at preparing novel functionalized SeNPs by NZ9000 through eco-friendly and economic biotechnology methods. Moreover, its physicochemical characteristics, antioxidant and anti-inflammatory activities were investigated. NZ9000 synthesized elemental red SeNPs when co-cultivated with sodium selenite under anaerobic conditions. Biosynthesized SeNPs by NZ9000 were mainly capped with polysaccharides and significantly alleviated the increase of malondialdehyde (MDA) concentration, the decrease of glutathione peroxidase (GPx) and total superoxide dismutase (T-SOD) activity in porcine intestinal epithelial cells (IPEC-J2) challenged by hydrogen peroxide (HO). SeNPs also prevented the HO-caused reduction of transepithelial electrical resistance (TEER) and the increase of FITC-Dextran fluxes across IPEC-J2. Moreover, SeNPs attenuated the increase of reactive oxygen species (ROS), the reduction of adenosine triphosphate (ATP) and the mitochondrial membrane potential (MMP) and maintained intestinal epithelial permeability in IPEC-J2 cells exposed to HO. In addition, SeNPs pretreatment alleviated the cytotoxicity of Enterotoxigenic (ETEC) K88 on IPEC-J2 cells and maintained the intestinal epithelial barrier integrity by up-regulating the expression of Occludin and Claudin-1 and modulating inflammatory cytokines. Biosynthesized SeNPs by NZ9000 are a promising selenium supplement with antioxidant and anti-inflammatory activities.

摘要

转基因的NZ9000是乳酸链球菌素调控基因表达最常用的宿主菌株。硒（Se）是人和动物饮食中的一种必需微量元素，对维持健康和生长很重要。以微生物为载体生物合成的硒纳米颗粒（SeNPs）在低成本、低毒性和高生物利用度方面具有独特优势。本研究旨在通过NZ9000采用生态友好且经济的生物技术方法制备新型功能化SeNPs。此外，还对其理化特性、抗氧化和抗炎活性进行了研究。在厌氧条件下，NZ9000与亚硒酸钠共培养时合成了元素红色SeNPs。NZ9000生物合成的SeNPs主要被多糖覆盖，显著减轻了过氧化氢（HO）攻击的猪肠上皮细胞（IPEC-J2）中丙二醛（MDA）浓度的升高、谷胱甘肽过氧化物酶（GPx）和总超氧化物歧化酶（T-SOD）活性的降低。SeNPs还防止了HO导致的跨上皮电阻（TEER）降低以及FITC-葡聚糖通过IPEC-J2的通量增加。此外，SeNPs减弱了活性氧（ROS）的增加、三磷酸腺苷（ATP）的减少和线粒体膜电位（MMP）的降低，并维持了暴露于HO的IPEC-J2细胞中的肠上皮通透性。此外，SeNPs预处理减轻了产肠毒素大肠杆菌（ETEC）K88对IPEC-J2细胞的细胞毒性，并通过上调闭合蛋白和紧密连接蛋白-1的表达以及调节炎性细胞因子来维持肠上皮屏障的完整性。NZ9000生物合成的SeNPs是一种有前景的具有抗氧化和抗炎活性的硒补充剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/114e/6676592/848d6eef3508/fmicb-10-01632-g007.jpg
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