伊朗中南部设拉子流浪猫中[具体内容缺失]的分子和血清学检测
Molecular and Serological Detection of in Stray Cats in Shiraz, South-central, Iran.
作者信息
Asgari Qasem, Mohammadpour Iraj, Pirzad Razieh, Kalantari Mohsen, Motazedian Mohammad Hossein, Naderi Shahrbanou
机构信息
Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Dept. of Medical Parasitology & Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Iran J Parasitol. 2018 Jul-Sep;13(3):430-439.
BACKGROUND
Toxoplasmosis is a global zoonotic disease that causes critical medical complications in neonates and immunocompromised persons. Infection rates in cats, specifically stray cats, are believed to be the best sentry of the level of in the environment. Therefore, in this study, we surveyed infection in stray cats of Shiraz, one of the metropolises of Iran.
METHODS
The appearance of antibodies and DNA of in samples from 145 stray cats was determined in order to appraise the prevalence of infection, by MAT and Nested-PCR.
RESULTS
The rate of infection in the cats was 69% by PCR and 82.8% by MAT. Besides, the highest rate of infection was discerned in diaphragm (37.9%) and intercostal muscle (34.5%), while the lowest rate was related to ileum (6.9%). Moreover, the similarity between MAT with titers 1:20, 1:40 and PCR were 79.2% and 86.2%, respectively (=0.02 and =0.0001).
CONCLUSION
Nested-PCR and MAT are valuable techniques for molecular and serological detection of . The prevalence of infection in stray cats in Shiraz is high.
背景
弓形虫病是一种全球性人畜共患病，可导致新生儿和免疫功能低下者出现严重的医学并发症。猫，特别是流浪猫的感染率被认为是环境中弓形虫水平的最佳哨兵。因此，在本研究中，我们调查了伊朗大城市之一设拉子流浪猫的弓形虫感染情况。
方法
通过微量凝集试验（MAT）和巢式聚合酶链反应（Nested-PCR）检测145只流浪猫样本中弓形虫抗体和DNA的出现情况，以评估弓形虫感染的患病率。
结果
通过PCR检测，猫的弓形虫感染率为69%，通过MAT检测为82.8%。此外，在膈肌（37.9%）和肋间肌（34.5%）中发现的感染率最高，而最低感染率与回肠（6.9%）有关。此外，MAT滴度为1:20、1:40时与PCR的相似度分别为79.2%和86.2%（P = 0.02和P = 0.0001）。
结论
巢式PCR和MAT是用于弓形虫分子和血清学检测的有价值技术。设拉子流浪猫的弓形虫感染患病率很高。
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