Norman Jeffrey S, King Gary M, Friesen Maren L
Plant Biology Department, Michigan State University, East Lansing, MI, USA.
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3597-3602. doi: 10.1099/ijsem.0.002175. Epub 2017 Sep 6.
Bacterial strain HPK2-2T was isolated from soil adjacent to the caldera of Kilauea Volcano in Hawaii Volcanoes National Park. HPK2-2T is a chemoorganoheterotroph that shows optimal growth at 50 °C (range 45-55 °C) and pH 8.0 (range 5.0-10.0). Sequence analysis of the 16S subunit of the rRNA gene showed that HPK2-2T is most closely related to the type strain of Rubrobactertaiwanensis (ATCC BAA-406T), with which it shared 94.5 % sequence identity. The major fatty acids detected in HPK2-2T were C18 : 0 14-methyl and C16 : 0 12-methyl; internally branched fatty acids such as these are characteristic of the genus Rubrobacter. The only respiratory quinone detected was MK-8, which is the major respiratory quinone for all members of the family Rubrobacteraceae examined thus far. We propose that HPK2-2T represents a novel species of the genus Rubrobacter, for which we propose the name Rubrobacterspartanus (type strain HPK2-2T; DSM 102139T; LMG 29988T).
细菌菌株HPK2-2T是从夏威夷火山国家公园基拉韦厄火山火山口附近的土壤中分离出来的。HPK2-2T是一种化能有机异养菌，在50°C（范围45-55°C）和pH 8.0（范围5.0-10.0）下生长最佳。rRNA基因16S亚基的序列分析表明，HPK2-2T与台湾红杆菌模式菌株（ATCC BAA-406T）关系最为密切，与其共享94.5%的序列同一性。在HPK2-2T中检测到的主要脂肪酸是C18:0 14-甲基和C16:0 12-甲基；像这样的内部分支脂肪酸是红杆菌属的特征。检测到的唯一呼吸醌是MK-8，这是迄今为止所检测的红杆菌科所有成员的主要呼吸醌。我们提议HPK2-2T代表红杆菌属的一个新物种，为此我们提议将其命名为斯巴坦红杆菌（模式菌株HPK2-2T；DSM 102139T；LMG 29988T）。
