Song Hanyi, Zhou Long, Liu Dongyan, Ge Lihui, Li Yan
Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.
Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.
Exp Ther Med. 2019 Sep;18(3):1551-1562. doi: 10.3892/etm.2019.7742. Epub 2019 Jul 5.
is a major cause of chronic gastritis, gastric ulcers and gastric cancer. Recent studies have identified that probiotics are beneficial to human health due, in part, to their anti- activities. Therefore, the present study investigated the antagonistic and local immunoregulatory activities of seven commercial probiotic strains and explored their mechanisms of actions. The human gastric epithelial cell line-1 (GES-1) was used to assess the effects of probiotics on the adhesion ability of . GES-1 cells were infected with plus lipopolysaccharide () or the drug-resistant strain () in the presence or absence of live probiotics. Following this, the growth rate and the adhesion ability of GES-1 cells were detected using MTT and urease activity assay. Toll-like receptor 4 (TLR4), NFKB inhibitor-α (IκBα) and nuclear factor (NF)-κB levels were measured by western blot analysis. The amount of interleukin (IL)-8 in the cell culture medium was determined by ELISA. Amongst the seven probiotic strains studied, live and inhibited adherence to GES-1 cells most significantly. inhibited IL-8 production by GES-1 cells through modulation of the TLR4/IκBα/NF-κB pathway. Therefore, the present results suggested that consumption of food containing and may be used as an adjuvant therapy for -associated gastritis.
是慢性胃炎、胃溃疡和胃癌的主要病因。最近的研究表明，益生菌对人体健康有益，部分原因在于它们的抗……活性。因此，本研究调查了七种市售益生菌菌株的拮抗和局部免疫调节活性，并探讨了它们的作用机制。用人胃上皮细胞系-1（GES-1）评估益生菌对……黏附能力的影响。在有或没有活的益生菌存在的情况下，用……加脂多糖（）或耐药……菌株（）感染GES-1细胞。随后，使用MTT和脲酶活性测定法检测GES-1细胞的生长速率和黏附能力。通过蛋白质印迹分析测量Toll样受体4（TLR4）、NFKB抑制因子-α（IκBα）和核因子（NF）-κB的水平。通过ELISA测定细胞培养基中白细胞介素（IL）-8的量。在所研究的七种益生菌菌株中，活的……和……最显著地抑制……对GES- cells的黏附。……通过调节TLR4/IκBα/NF-κB途径抑制GES-1细胞产生IL-8。因此，目前的结果表明，食用含有……和……的食物可作为与……相关胃炎的辅助治疗方法。
