Rotmans J, Duchenne W, Linschooten C, In't Veld N, Polderman A
Laboratory of Parasitology, University of Leiden, Medical Faculty, The Netherlands.
Eur J Clin Microbiol Infect Dis. 1988 Aug;7(4):535-8. doi: 10.1007/BF01962609.
Immunoglobulin M antibodies against a major serological antigen (Mr 6 kD) were detected in human sera by indirect ELISA, antibody capture ELISA, and immunoblot test. In contrast to indirect ELISA, the immunoblot test gave no false positive reactions, not even with those sera containing a high level of rheumatoid factor. However, the immunoblot test gave false negative results with sera which gave positive results in both ELISA tests. The antibody capture ELISA gave no false negative reactions. All positive sera except one reacted specifically with Toxoplasma gondii antigens.
通过间接酶联免疫吸附测定（ELISA）、抗体捕获ELISA和免疫印迹试验在人血清中检测到针对一种主要血清学抗原（分子量6千道尔顿）的免疫球蛋白M抗体。与间接ELISA不同，免疫印迹试验未出现假阳性反应，即使是那些含有高水平类风湿因子的血清也未出现。然而，免疫印迹试验对在两种ELISA试验中均呈阳性的血清给出了假阴性结果。抗体捕获ELISA未出现假阴性反应。除一份血清外，所有阳性血清均与刚地弓形虫抗原发生特异性反应。
