Ruitenberg E J, van Knapen F
J Infect Dis. 1977 Oct;136 Suppl:S267-73. doi: 10.1093/infdis/136.supplement_2.s267.
The enzyme-linked immunosorbent assay (ELISA) was used for the serodiagnosis of four parasitic infections. ELISA was performed as a macromethod in polystyrene tubes and/or as a micromethod in polystyrene microplates. Conventionally raised pigs were infected orally with different numbers of Trichinealla spiralis larvae, and the pigs' sera were tested for antibody to T. spiralis. Results were compared with those from sera of uninfected conventionally raised pigs. Cynomolgus monkeys were infected orally and immunized with Toxocara canis. The results of macro-ELISA and micro-ELISA were compared with those of immunofluorescence. Micro-ELISA proved to be more sensitive than macro-ELISA, the latter being more sensitive than immunofluorescence. The applicability of ELISA for seroepidemiology was demonstrated by results obtained with sera from patients with proved African trypanosomiasis. With both macro-ELISA and micro-ELISA it was possible to discriminate between the positive and negative sera. ELISA was used for study of the seroepidemiology of toxoplasmosis. Again, positive sera could be distinguished from negative sera. The examples presented show that ELISA can be used as an additional serologic tool for the detection of both helminthic and protozoal infections.
酶联免疫吸附测定（ELISA）用于四种寄生虫感染的血清学诊断。ELISA以大分子方法在聚苯乙烯管中进行，和/或以小分子方法在聚苯乙烯微孔板中进行。常规饲养的猪经口感染不同数量的旋毛虫幼虫，检测猪血清中的抗旋毛虫抗体。将结果与未感染的常规饲养猪的血清结果进行比较。食蟹猴经口感染犬弓首蛔虫并进行免疫。将大分子ELISA和小分子ELISA的结果与免疫荧光结果进行比较。结果表明，小分子ELISA比大分子ELISA更灵敏，而大分子ELISA比免疫荧光更灵敏。通过对确诊为非洲锥虫病患者血清的检测结果，证明了ELISA在血清流行病学中的适用性。使用大分子ELISA和小分子ELISA均可区分阳性和阴性血清。ELISA用于弓形虫病血清流行病学研究。同样，阳性血清也可与阴性血清区分开来。所举实例表明，ELISA可作为检测蠕虫感染和原生动物感染的一种额外血清学工具。
