用于检测抗弓形虫IgG的快速间接酶联免疫吸附测定（ELISA）：与染色试验的比较
Rapid indirect enzyme linked immunosorbent assay (ELISA) for detecting antitoxoplasma IgG: comparison with dye test.
作者信息
Francis J M, Payne R A, Joynson D H
机构信息
Public Health Laboratory, Singleton Hospital, Swansea.
出版信息
J Clin Pathol. 1988 Jul;41(7):802-5. doi: 10.1136/jcp.41.7.802.
Abstract
A rapid and simple enzyme linked immunosorbent assay (ELISA) for the detection of specific IgG against Toxoplasma gondii was compared with the dye test on 533 serum samples. In general, results were comparable but not with sera that contained high concentrations of toxoplasma specific IgM or that had been heated at 56 degrees C. There were no false positive results with sera containing rheumatoid factor or anti-nuclear factor. It is concluded that if a dye test is not to be performed then the serum should be tested for both toxoplasma specific IgG and IgM to avoid misleading results. Heat inactivated serum should also not be tested in this type of specific IgG assay.
摘要
将一种用于检测抗弓形虫特异性IgG的快速简便的酶联免疫吸附测定（ELISA）法与533份血清样本的染色试验进行了比较。总体而言，结果具有可比性，但含有高浓度弓形虫特异性IgM的血清或经56℃加热的血清除外。含类风湿因子或抗核因子的血清未出现假阳性结果。得出的结论是，如果不进行染色试验，那么血清应同时检测弓形虫特异性IgG和IgM，以避免产生误导性结果。热灭活血清也不应在此类特异性IgG测定中进行检测。
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