Department of Otolaryngology-Head and Neck Surgery, The Second Hospital of Jilin University, 218 Ziqiang Street, Changchun, 130041, China.
Department of Otolaryngology-Head and Neck Surgery, The Central Hospital of Wuhan, 26 Shengli Street, Wuhan, 430014, China.
Sci Rep. 2019 Aug 14;9(1):11823. doi: 10.1038/s41598-019-47895-x.
Choline phosphate-based delivery systems can target the acidic tumor microenvironment. In this study, we set out to evaluate the diagnostic value of Choline phosphate cytidylyltransferase-α (CCTα) in laryngeal squamous cell cancer (LSCC). The expression of CCTα was detected using immunohistochemistry in 50 LSCC patients' tissues and 16 vocal polyps as control group. Then, clinical data was collected and we used receiver operating characteristic curve (ROC) to estimate the potential of CCTα as diagnostic biomarker. We found CCTα levels to be significantly high in the tissues derived from LSCC patients, (p < 0.001). Further, we observed a positive correlation of CCTα with tumor size (p < 0.001), TNM stage (p < 0.001), lymph node metastasis (p < 0.001) as well as the grade of LSCC malignancy (p < 0.001). Furthermore, AUC was determined to be 0.939 by ROC, and the optimal cutoff value 3.100, with 76.0% sensitivity and 100% specificity. We also found an epigenetic basis of CCTα over-expression in LSCC tissues with significantly reduced methylation of CCTα in LSCC tissues, compared to vocal polyps (p < 0.001). These results support epigenetically-induced over-expression of CCTα as a potential diagnostic marker for LSCC.
基于磷酸胆碱的递药系统可以靶向酸性肿瘤微环境。在本研究中，我们旨在评估磷酸胆碱胞苷酰转移酶-α（CCTα）在喉鳞状细胞癌（LSCC）中的诊断价值。采用免疫组织化学法检测 50 例 LSCC 患者组织和 16 例声带息肉（对照组）中 CCTα 的表达。然后收集临床资料，采用受试者工作特征曲线（ROC）估计 CCTα 作为诊断生物标志物的潜力。结果发现，LSCC 患者组织中 CCTα 水平显著升高（p<0.001）。进一步观察到 CCTα 与肿瘤大小（p<0.001）、TNM 分期（p<0.001）、淋巴结转移（p<0.001）以及 LSCC 恶性程度分级（p<0.001）呈正相关。ROC 曲线确定 AUC 为 0.939，最佳截断值为 3.100，灵敏度为 76.0%，特异性为 100%。我们还发现 LSCC 组织中 CCTα 表达的表观遗传学基础，与声带息肉相比，LSCC 组织中 CCTα 的甲基化显著降低（p<0.001）。这些结果支持 CCTα 的表观遗传诱导过表达作为 LSCC 的潜在诊断标志物。
