Eliasse Yoan, Galliano Marie-Florence, Redoules Daniel, Espinosa Eric
INSERM U1037, Centre de Recherche en Cancérologie de Toulouse (CRCT), Toulouse F-31037, France.
Université De Toulouse, Université Paul Sabatier, Toulouse F-31062, France.
J Inflamm Res. 2019 Jul 22;12:181-194. doi: 10.2147/JIR.S213594. eCollection 2019.
Hydrotherapy appears as a valuable therapeutic tool in the management of patients suffering from chronic skin inflammatory diseases. Nevertheless, the underlying immune mechanisms of these beneficial effects remain poorly understood. To better understand the biological effects of thermal spring water on the immune system, we investigated the effects of Avène thermal spring water (ASW) on dendritic cells as key cells participating in the control of the immune response.
Dendritic cells (DCs) were generated from human monocytes and matured with LPS in ASW-based culture medium or in dexamethasone supplemented culture medium as an anti-inflammatory treatment. The phenotypes and abilities of these DCs to produce cytokines and induce allogeneic T cell response was next assessed.
We showed that ASW modulated the differentiation of monocytes into DCs and impacted the DC maturation upon LPS priming. We observed a reduction of the CD83, CD86, CD1a and HLA-DR molecule expression and a decrease of IL-12 and IL-23 production whereas IL-10 production was increased. LPS-primed DCs generated in presence of ASW exhibited a reduced capacity to induce naive CD4 T cell proliferation and IFN-γ and IL-17 production.
Our study showed that ASW is endowed with an immunomodulatory potential. ASW limits the DC stimulatory capacity of Th1 and Th17 cell responses by impairing their maturation, IL-12 and IL-23 production and accessory cell function.
水疗法似乎是治疗慢性皮肤炎症性疾病患者的一种有价值的治疗工具。然而，这些有益效果的潜在免疫机制仍知之甚少。为了更好地理解温泉水对免疫系统的生物学效应，我们研究了雅漾温泉水（ASW）对作为参与免疫反应控制的关键细胞的树突状细胞的影响。
从人单核细胞生成树突状细胞（DCs），并在基于ASW的培养基或补充地塞米松的培养基中用脂多糖（LPS）使其成熟，作为一种抗炎治疗。接下来评估这些DCs产生细胞因子和诱导同种异体T细胞反应的表型和能力。
我们表明，ASW调节单核细胞向DCs的分化，并影响LPS启动后的DC成熟。我们观察到CD83、CD86、CD1a和HLA-DR分子表达减少，IL-12和IL-23产生减少，而IL-10产生增加。在ASW存在下产生的LPS启动的DCs诱导初始CD4 T细胞增殖以及IFN-γ和IL-17产生的能力降低。
我们的研究表明，ASW具有免疫调节潜力。ASW通过损害Th1和Th17细胞反应的DC刺激能力、它们的成熟、IL-12和IL-23产生以及辅助细胞功能来限制这种能力。
