Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Korea.
Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Korea.
Int J Mol Sci. 2019 Aug 14;20(16):3958. doi: 10.3390/ijms20163958.
We fabricated poly (ethylene glycol)-block-polycaprolactone (PEG-b-PCL) nanoemulsion for drug delivery and photodynamic therapy. PEG-b-PCL effectively stabilized the interface between water and soybean oil, and the resulting nanoemulsion was about 220.3 nm in diameter with spherical shape. For photodynamic therapy (PDT), chlorin e6 (Ce6) was loaded into the nanoemulsion as a photosensitizer (PS). These chlorin e6-loaded PEG-PCL nanoemulsions (Ce6-PCL-NEs) showed efficient cellular uptake and, upon laser irradiation, generated singlet oxygen to kill tumor cells. Particularly, Ce6-PCL-NEs showed prolonged blood circulation and about 60% increased tumor accumulation compared to free Ce6 after intravenous injection to 4T1 tumor-bearing mice. These results demonstrate the promising potential of Ce6-PCL-NEs for efficient PDT and in vivo drug delivery to tumor tissue.
我们制备了聚乙二醇-嵌段-聚己内酯（PEG-b-PCL）纳米乳液用于药物输送和光动力疗法。PEG-b-PCL 有效地稳定了水和大豆油之间的界面，所得纳米乳液的直径约为 220.3nm，呈球形。对于光动力疗法（PDT），将叶绿素 e6（Ce6）负载到纳米乳液中作为光敏剂（PS）。这些载有叶绿素 e6 的 PEG-PCL 纳米乳液（Ce6-PCL-NEs）表现出高效的细胞摄取能力，并且在激光照射下产生单线态氧来杀死肿瘤细胞。特别地，与静脉注射到 4T1 荷瘤小鼠的游离 Ce6 相比，Ce6-PCL-NEs 显示出延长的血液循环时间和约 60%的肿瘤蓄积增加。这些结果表明 Ce6-PCL-NEs 具有用于有效 PDT 和向肿瘤组织体内递药的有前途的潜力。
