非洲爪蟾转录因子TFIIIA的基因结构。
Structure of the gene for Xenopus transcription factor TFIIIA.
作者信息
Tso J Y, Van Den Berg D J, Korn L J
出版信息
Nucleic Acids Res. 1986 Mar 11;14(5):2187-200. doi: 10.1093/nar/14.5.2187.
The eucaryotic transcription factor TFIIIA is required for 5S RNA transcription in Xenopus, and changes in the level of TFIIIA have been implicated in the differential expression of 5S RNA genes. In this paper, we report the isolation and sequencing of the X. laevis TFIIIA gene. The gene is approximately 11 kb in length and consists of 9 coding segments separated by 8 introns. A sequence of 30 amino acid residues is known to repeat imperfectly 9 times in tandem within the TFIIIA protein, and Miller et al. (EMBO J. 4, 1609-1614, 1985) proposed that TFIIIA evolved by duplication of a primordial 30 amino acid residue unit. Our results from DNA sequence analysis support their proposal by showing that some of the exon-intron boundaries correspond closely to the repeating unit. We also found that the 5' flanking sequence of the TFIIIA gene contains a TATA box (TATATAA) at position -32 and a CAAT box (GCCAATCC) at position -96 and that the site of polyadenylation is 255 residues 3' of the stop codon. Finally, we have shown that the coding sequence of the TFIIIA gene is significantly polymorphic.
真核转录因子TFIIIA是非洲爪蟾5S RNA转录所必需的，并且TFIIIA水平的变化与5S RNA基因的差异表达有关。在本文中，我们报道了非洲爪蟾TFIIIA基因的分离和测序。该基因长度约为11 kb，由9个编码区段组成，被8个内含子隔开。已知一段30个氨基酸残基的序列在TFIIIA蛋白中串联不完全重复9次，并且米勒等人（《欧洲分子生物学组织杂志》4，1609 - 1614，1985）提出TFIIIA是由一个原始的30个氨基酸残基单元重复复制进化而来的。我们DNA序列分析的结果通过表明一些外显子 - 内含子边界与重复单元紧密对应来支持他们的提议。我们还发现TFIIIA基因的5'侧翼序列在 - 32位含有一个TATA框（TATATAA），在 - 96位含有一个CAAT框（GCCAATCC），并且聚腺苷酸化位点在终止密码子下游255个残基处。最后，我们表明TFIIIA基因的编码序列具有显著的多态性。
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