Pharmacy College, Federal University of Bahia, UFBA, Salvador-BA 40170-115, Brazil.
Faculty of Technology and Sciences of Bahia, FATEC-BA, Salvador-BA 40280-901, Brazil.
Biomed Res Int. 2019 Jul 24;2019:7523670. doi: 10.1155/2019/7523670. eCollection 2019.
Epidemiological studies on species-specific infections are scarce due to the morphological similarity of pathogenic and nonpathogenic and . The diagnosis of is frequently based on coproantigen (-Gal/GalNAc lectin specific) detection by immunoassays. However, specific -lectin is not expressed in cysts, which are eliminated by asymptomatic individuals leading to false-negative results and an underestimation of amebiasis prevalence. Molecular techniques based on the amplification of parasite DNA have been shown to be a highly sensitive and specific method that allows the detection of different species. This study aimed to assess the frequency of the species from complex by molecular and immunological techniques in individuals attended at a public health system in Salvador-Bahia, Brazil. A cross-sectional study involving 55,218 individuals was carried out. The diagnosis was based on microscopy revealing complex. The species differentiation was performed by -specific antigen, serological evaluation and by molecular technique. The overall prevalence of complex determined by microscopy was approximately 0.49% (273/55,218). -specific antigen detection and molecular characterization returned 100% negativity for . However, serological evaluation returned an 8.9% positivity (8/90). In the stool samples analysed by PCR, it was not possible to identify and , although circulating IgG anti- has been detected.
由于致病性和非致病性 和 的形态相似，针对物种特异性感染的流行病学研究较为匮乏。 的诊断通常基于免疫检测法对粪抗原（Gal/GalNAc 凝集素特异性）的检测。然而，在无症状个体中，囊包会被排出，导致特定的 - 凝集素无法被检测到，从而产生假阴性结果，并低估了阿米巴病的流行率。基于寄生虫 DNA 扩增的分子技术已被证明是一种高度敏感和特异的方法，可用于检测不同的 物种。本研究旨在通过分子和免疫技术评估巴西萨尔瓦多-巴伊亚公共卫生系统就诊个体中 复合体的物种频率。进行了一项涉及 55218 人的横断面研究。诊断基于显示 复合体的显微镜检查。通过 - 特异性抗原、血清学评估和分子技术进行物种分化。通过显微镜确定的 复合体总体流行率约为 0.49%（273/55218）。- 特异性抗原检测和分子特征分析对 呈 100%阴性，但血清学评估呈 8.9%阳性（8/90）。在通过 PCR 分析的粪便样本中，虽然检测到循环 IgG 抗-，但无法识别 和 。
