Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior , Ciudad Universitaria , Coyoacán 04510 , Cd. Mx , Mexico.
Facultad de Ciencias Naturales , Universidad Autónoma de Querétaro , Avenida de las Ciencias s/n, Col. Juriquilla , 76230 , Qro , Querétaro , Mexico.
J Nat Prod. 2019 Sep 27;82(9):2489-2500. doi: 10.1021/acs.jnatprod.9b00233. Epub 2019 Aug 20.
Chemical investigation of the aerial parts (except fruits and calixes) of var. led to the isolation of a series of new labdane-type diterpenoids, including the closely related compounds -, the labdane glucosides and , a mixture of the epimeric alcohols and , and one labdanetriol, isolated as its tri--acetyl derivative . In addition, three new withanolides (-) and six known compounds were isolated. The structures of these compounds were elucidated by analysis of their spectroscopic data and chemical transformations, and those of compounds , , and were confirmed by X-ray diffraction analysis of the natural product () and of the corresponding acetyl derivatives and . Fourteen of these compounds were assayed for their in vitro inhibitory activity against yeast α-glucosidase and acetylcholinesterase enzymes. The results were negative in both cases, except for compound that marginally inhibited the activity of acetylcholinesterase with an IC value of 64.4 μM.
对 变种的地上部分（果实和花萼除外）的化学研究导致了一系列新型的贝壳杉烷型二萜的分离，包括密切相关的化合物-、贝壳杉烷糖苷-和-、互为差向异构体的醇-和-的混合物，以及作为其三乙酰衍生物分离出的一个贝壳杉烷三醇。此外，还分离出三种新的羽扇豆烷内酯（-）和六种已知化合物。通过分析它们的光谱数据和化学转化，这些化合物的结构得到了阐明，化合物-、-和-的结构通过天然产物（）及其相应的乙酰衍生物（）的 X 射线衍射分析得到了证实。对其中的 14 种化合物进行了体外抑制酵母α-葡萄糖苷酶和乙酰胆碱酯酶活性的测定。两种情况下的结果均为阴性，只有化合物-对乙酰胆碱酯酶的活性有轻微抑制作用，IC 值为 64.4 μM。
