Johnstone I B, Crane S
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph.
Can J Vet Res. 1988 Oct;52(4):416-8.
The vasopressin analog 1-desamino-8-D-arginine stimulates elevations in plasma Factor VIII/ von Willebrand factor in normal dogs. In order to study the effects of general anesthesia on this response, six dogs were anesthetized with sodium pentobarbital or given an equivalent amount of saline then challenged with an intravenous dose of 1-desamino-8-D-arginine (0.6 micrograms/kg body weight). Factor VIII coagulant activity, von Willebrand factor antigen, and ristocetin cofactor activity were quantitated before anesthesia (or saline infusion), 20 min after induction (pre-1-desamino-8-D-arginine), and at 30 and 60 min post-1-desamino-8-D-arginine. Anesthesia did not significantly affect the elevations in plasma Factor VIII/ von Willebrand factor induced by 1-desamino-8-D-arginine. Sodium pentobarbital appeared however to prevent the rise in Factor VIII coagulant activity seen following saline treatment. The results of this study suggest that when 1-desamino-8-D-arginine is to be used in normal dogs to boost basal plasma von Willebrand factor levels, it is not necessary to administer it prior to induction of general anesthesia with sodium pentobarbital.
血管加压素类似物1-去氨基-8-D-精氨酸可刺激正常犬血浆中因子VIII/血管性血友病因子升高。为研究全身麻醉对该反应的影响，6只犬用戊巴比妥钠麻醉或给予等量生理盐水，然后静脉注射1-去氨基-8-D-精氨酸（0.6微克/千克体重）进行激发试验。在麻醉前（或生理盐水输注前）、诱导后20分钟（1-去氨基-8-D-精氨酸给药前）以及1-去氨基-8-D-精氨酸给药后30分钟和60分钟，对因子VIII凝血活性、血管性血友病因子抗原和瑞斯托霉素辅因子活性进行定量检测。麻醉对1-去氨基-8-D-精氨酸诱导的血浆因子VIII/血管性血友病因子升高无显著影响。然而，戊巴比妥钠似乎可阻止生理盐水处理后出现的因子VIII凝血活性升高。本研究结果表明，当在正常犬中使用1-去氨基-8-D-精氨酸提高基础血浆血管性血友病因子水平时，在使用戊巴比妥钠诱导全身麻醉前给药并非必要。
