Institute of Health and Care Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Section for Ophthalmology, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Front Immunol. 2020 Nov 3;11:597632. doi: 10.3389/fimmu.2020.597632. eCollection 2020.
Biomarker analysis allows for the detection and prediction of disease as well as health monitoring. The use of interstitial fluid (ISF) as a matrix for biomarkers has recently gained interest. This study aimed to compare levels of inflammatory markers in ISF from suction blister fluid (SBF) and plasma.
Plasma and SBF were collected from 18 healthy individuals. Samples were analyzed for 92 inflammation-related protein biomarkers by Proximity Extension Assay (PEA). Protein profiles in the two matrices were compared using traditional and multivariate statistics.
Out of 92 targeted proteins, 70 were successfully quantified in both plasma and SBF. Overall, plasma and SBF displayed distinct protein profiles with up to 40-fold difference in abundance of specific proteins. The levels of 25 proteins were significantly correlated between plasma and SBF and several of these were recognized as potential markers to monitor health using ISF.
Skin ISF and plasma have unique protein profiles but many inflammatory markers are proportionally related between the matrices at the individual level. ISF is a promising biofluid for the monitoring of biomarkers in clinical studies and routine analyses.
生物标志物分析可用于检测和预测疾病以及健康监测。最近，人们对将细胞间液（ISF）作为生物标志物基质的应用产生了兴趣。本研究旨在比较抽吸水疱液（SBF）和血浆中炎症标志物的水平。
从 18 名健康个体中采集血浆和 SBF。通过接近延伸分析（PEA）对 92 种与炎症相关的蛋白生物标志物进行分析。使用传统和多变量统计学方法比较两种基质中的蛋白谱。
在 92 种靶向蛋白中，有 70 种在血浆和 SBF 中均成功定量。总体而言，血浆和 SBF 显示出独特的蛋白谱，某些蛋白的丰度差异高达 40 倍。血浆和 SBF 之间有 25 种蛋白的水平存在显著相关性，其中一些被认为是使用 ISF 监测健康的潜在标志物。
皮肤 ISF 和血浆具有独特的蛋白谱，但在个体水平上，许多炎症标志物在两种基质之间呈比例相关。ISF 是临床研究和常规分析中监测生物标志物的有前途的生物流体。
