Cui Qingjun
Department of Laboratory, Xintai People's Hospital, Xintai, Shandong 271200, P.R. China.
Oncol Lett. 2019 Sep;18(3):3092-3096. doi: 10.3892/ol.2019.10621. Epub 2019 Jul 16.
Clinical significance of micro-ribonucleic acid (miR)-27a and miR-31 in the early diagnosis and prognosis of colorectal cancer were investigated. Forty patients with colorectal malignancy admitted to Xintai People's Hospital from February 2014 to April 2018 were enrolled as the observation group, of which 30 patients were diagnosed via pathological biopsy. Another 40 patients diagnosed with colorectal polyp and receiving surgical treatment were selected as the control group. The relative amount of miR-27a and miR-31 was measured. The relative expression levels of miR-27a and miR-31 in patients were analyzed. The diagnostic sensitivity, specificity and accordance rates of positive miR-27a and miR-31 expression in colorectal cancer were recorded. The correlation of the relative expression levels of miR-27a and miR-31 with the survival time of patients were analyzed. In the observation group, the relative expression levels of miR-27a and miR-31 in patients with lymph node metastasis and distant metastasis were higher than those in patients without lymph node metastasis and distant metastasis (P<0.05). Histological type of patients with non-mucinous carcinoma had increased relative expression levels of miR-27a and miR-31 in comparison with those with mucinous carcinoma (P<0.05). In terms of Duke's grade, the relative expression levels of miR-27a and miR-31 in patients with grade C and D were higher than those in patients with grade A and B (P<0.05). The diagnostic sensitivity, specificity and accordance rate of positive miR-27a expression were lower than those of positive miR-31 expression. The relative expression levels of miR-27a and miR-31 were positively correlated with the survival time of patients (P<0.05). The expression levels of miR-27a and miR-31 are related to distant metastasis and tumor grade of patients with colorectal cancer, and positively associated with the survival time of patients, having high diagnostic value.
研究了微小核糖核酸（miR）-27a和miR-31在结直肠癌早期诊断及预后中的临床意义。选取2014年2月至2018年4月新泰市人民医院收治的40例结直肠恶性肿瘤患者作为观察组，其中30例经病理活检确诊。另选取40例诊断为结直肠息肉并接受手术治疗的患者作为对照组。检测miR-27a和miR-31的相对含量。分析患者中miR-27a和miR-31的相对表达水平。记录miR-27a和miR-31阳性表达在结直肠癌中的诊断敏感性、特异性及符合率。分析miR-27a和miR-31相对表达水平与患者生存时间的相关性。观察组中，有淋巴结转移和远处转移患者的miR-27a和miR-31相对表达水平高于无淋巴结转移和远处转移患者（P<0.05）。非黏液腺癌患者的miR-27a和miR-31相对表达水平高于黏液腺癌患者（P<0.05）。在杜克分期方面，C期和D期患者的miR-27a和miR-31相对表达水平高于A期和B期患者（P<0.05）。miR-27a阳性表达的诊断敏感性、特异性及符合率低于miR-31阳性表达。miR-27a和miR-31的相对表达水平与患者生存时间呈正相关（P<0.05）。miR-27a和miR-31的表达水平与结直肠癌患者的远处转移及肿瘤分级有关，且与患者生存时间呈正相关，具有较高的诊断价值。
