Chen Xin-Yue, Gou San-Hu, Shi Zhi-Qiang, Xue Zhi-Yuan, Feng Shi-Lan
School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
J Pharm Anal. 2019 Aug;9(4):266-273. doi: 10.1016/j.jpha.2018.10.004. Epub 2018 Nov 1.
The traditional Chinese medicine of plays an important role in invigorating gas for ascending, benefiting blood for promoting production of fluid, and promoting circulation for removing obstruction in collaterals, which is consistent with the principle of treatment for osteoporosis. This study is designed to investigate the bioactive components on increasing peak bone mass (PBM) by exploring the spectrum-effect relationship between chromatography fingerprints and effect. Multiple indicators are selected to evaluate the pharmacological activity. In fingerprints, 21 common peaks are obtained, five of which are identified. Furthermore, gray relational analysis (GRA) is a quantitative method of gray system theory and is used to describe the correlation degree of common peaks and pharmacological activities with relational value. 21 components are then divided into three different regions, of which ononin and calycosin play an extremely significant role in increasing PBM. In addition, factor analysis and hierarchical cluster analysis (HCA) are used to screen the optimal producing area for . This provides a comprehensive and efficient method to improve the quality evaluation of , confirming the bioactive components for PBM-enhancement and further develop its medicinal value.
中药在益气升提、补血生液、活血通络方面发挥着重要作用，这与骨质疏松症的治疗原则相符。本研究旨在通过探索色谱指纹图谱与效应之间的谱效关系，研究增加峰值骨量（PBM）的生物活性成分。选择多个指标来评估药理活性。在指纹图谱中，获得了21个共有峰，其中5个已被鉴定。此外，灰色关联分析（GRA）是灰色系统理论的一种定量方法，用于用关联度描述共有峰与药理活性之间的相关性。然后将21种成分分为三个不同区域，其中芒柄花苷和毛蕊异黄酮在增加PBM方面发挥着极其显著的作用。此外，采用因子分析和层次聚类分析（HCA）筛选[中药名称]的最佳产地。这为提高[中药名称]的质量评价提供了一种全面有效的方法，确定了增强PBM的生物活性成分，并进一步开发其药用价值。
