School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China.
Molecules. 2019 Aug 26;24(17):3092. doi: 10.3390/molecules24173092.
Tartary buckwheat ( (L.) Gaertn) is rich in functional compounds such as rutin, quercetin, d-chiro-inositol, dietary fiber, and essential amino acids. Electric field (EF) treatment before sprout germination results in physiological and chemical changes, and some alterations might lead to positive applications in plant seeds. MTT assay showed that the effect of total flavonoids on human gastric cancer cell line MGC80-3 was significantly changed after EF treatment for different germination days (3-7 days). Among them, the total flavonoids of tartary buckwheat (BWTF) on the third day had the most obvious inhibitory effect on MGC80-3 ( < 0.01). In addition, flow cytometry evidenced that different ratios of quercetin and rutin had effects on the proliferation of MGC80-3. The same content of quercetin and rutin had the best effect, reaching 6.18 ± 0.82%. The anti-cancer mechanism was mainly promoted by promoting the expression of apoptotic proteins. The expression of Bax/Bcl-2 and caspase-8 in MGC80-3 cells was mediated by BWTFs. This study has good research value for improving the biological and economic value of tartary buckwheat.
鞑靼荞麦（（L.）Gaertn）富含芦丁、槲皮素、D-手性肌醇、膳食纤维和必需氨基酸等功能性化合物。电场（EF）处理发芽前会导致生理和化学变化，一些变化可能会导致植物种子的积极应用。MTT 检测表明，在不同发芽天数（3-7 天）进行 EF 处理后，总黄酮对人胃癌细胞系 MGC80-3 的作用明显改变。其中，荞麦总黄酮（BWTF）在第三天对 MGC80-3 的抑制作用最明显（<0.01）。此外，流式细胞术证实，不同比例的槲皮素和芦丁对 MGC80-3 的增殖有影响。相同含量的槲皮素和芦丁效果最好，达到 6.18±0.82%。抗癌机制主要通过促进凋亡蛋白的表达来促进。BWTFs 介导了 MGC80-3 细胞中 Bax/Bcl-2 和 caspase-8 的表达。本研究对提高鞑靼荞麦的生物学和经济价值具有很好的研究价值。
