Department of Clinical Sciences, Division of Infectious Diseases, University of Agricultural Sciences and Veterinary Medicine, 400374 Cluj-Napoca, Romania.
Department of Pharmacognosy, "Iuliu Haţieganu" University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania.
Molecules. 2019 Aug 26;24(17):3101. doi: 10.3390/molecules24173101.
Willd. is a Lamiaceae species spread in a large variety of habitats worldwide. The aim of the present research was to analyse two different samples belonging to this species, one obtained from the spontaneous flora and one from culture. The total polyphenols, flavonoids, and phenolic acid contents were spectrophotometrically determined. Qualitative and quantitative analysis of polyphenols was performed by an HPLC-DAD-ESI (+)-MS method. For the antibacterial assay, the well-diffusion and the broth microdilution methods were used. Analysis of polyphenols revealed for both samples the presence of flavonoids like luteolin, quercetin, apigenin and their derivatives, but also of rosmarinic acid and methyl-rosmarinate. Differences regarding the amount of these compounds were emphasized. Significantly larger amounts of flavonoids were found for the sample harvested in the spontaneous flora, while for the rosmarinic acid, larger amounts were found for the cultured sample. Both samples displayed promising antibacterial activity, particularly towards Gram positive organisms. represents, therefore, a rich source of polyphenolic compounds that prove its promising potential as a medicinal species.
Willd. 是唇形科的一个物种，分布于世界各地的多种生境中。本研究的目的是分析属于该物种的两个不同样本，一个是从自然植物群中获得的，另一个是从培养中获得的。采用分光光度法测定总多酚、类黄酮和酚酸含量。采用 HPLC-DAD-ESI (+)-MS 方法对多酚进行定性和定量分析。采用平板扩散法和肉汤微量稀释法进行抑菌试验。多酚分析表明，两个样本都含有类黄酮，如木樨草素、槲皮素、芹菜素及其衍生物，还有迷迭香酸和甲基迷迭香酸。这些化合物的含量存在差异。从自然植物群中采集的样本中发现了大量的类黄酮，而对于迷迭香酸，培养样本中的含量更大。两个样本都表现出了有前景的抗菌活性，特别是对革兰氏阳性菌。因此，代表了一个富含多酚化合物的来源，证明了它作为药用物种的有前途的潜力。
