Dervişoğlu Gökhan, Kaya Bülent, Aykutoğlu Gürkan, Darendelioğlu Ekrem, Ayna Adnan
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bingöl University, 12000, Bingol, Türkiye.
Department of Chemistry, Faculty of Arts and Sciences, Bingöl University, 12000, Bingol, Türkiye.
Mol Biol Rep. 2025 Aug 8;52(1):811. doi: 10.1007/s11033-025-10924-2.
Silver nanoparticles (AgNPs) have garnered great attention due to their biocompatibility and environmental safety. However, no prior study has focused on the synthesis, characterization, and biological evaluation of AgNPs prepared with Bingol propolis (PrAgNPs).
PrAgNPs were synthesized using a green chemistry approach and characterized by UV-Vis spectroscopy, dynamic light scattering (DLS), zeta potential, X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). XRD analysis confirmed the presence of predominantly cubic crystals, and TEM analysis revealed an average particle size of 14.45 ± 3.44 nm. Zeta potential measurements indicated a negative surface charge of - 24.6 mV, suggesting good colloidal stability. LC-MS analysis identified acacetin and etoposide as key phenolic compounds in the ethanol extract of propolis (EEP). The antioxidant activity of PrAgNPs was assessed by DPPH and hydroxyl radical scavenging assays, showing significantly higher activity compared to EEP. The cytotoxicity and anticancer potential were evaluated in the PC-3 prostate cancer cell line using the WST-1 assay and Western blot analysis. PrAgNPs effectively inhibited cell proliferation and induced apoptosis as evidenced by elevated levels of cytochrome c and pro-caspase-3 proteins.
This study demonstrated that PrAgNPs, synthesized by an environmentally friendly route, possess potent antioxidant and anti-cancer activities. These findings highlight the promising therapeutic potential of PrAgNPs for future applications in cancer treatment.
银纳米颗粒（AgNPs）因其生物相容性和环境安全性而备受关注。然而，此前尚无研究聚焦于用宾戈尔蜂胶制备的银纳米颗粒（PrAgNPs）的合成、表征及生物学评价。
采用绿色化学方法合成PrAgNPs，并通过紫外可见光谱、动态光散射（DLS）、zeta电位、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和透射电子显微镜（TEM）进行表征。XRD分析证实主要存在立方晶体，TEM分析显示平均粒径为14.45±3.44纳米。zeta电位测量表明表面电荷为-24.6毫伏，表明具有良好的胶体稳定性。液相色谱-质谱分析确定刺槐素和依托泊苷为蜂胶乙醇提取物（EEP）中的关键酚类化合物。通过DPPH和羟基自由基清除试验评估PrAgNPs的抗氧化活性，结果显示其活性显著高于EEP。使用WST-1试验和蛋白质印迹分析在PC-3前列腺癌细胞系中评估细胞毒性和抗癌潜力。PrAgNPs有效抑制细胞增殖并诱导凋亡，细胞色素c和前半胱天冬酶-3蛋白水平升高证明了这一点。
本研究表明，通过环境友好途径合成的PrAgNPs具有强大的抗氧化和抗癌活性。这些发现突出了PrAgNPs在未来癌症治疗应用中的广阔治疗潜力。
