Institute of Urology and Nephrology.
Center for Genomic and Personalized Medicine.
J Am Soc Nephrol. 2019 Nov;30(11):2159-2176. doi: 10.1681/ASN.2019040335. Epub 2019 Aug 28.
Having a comprehensive map of the cellular anatomy of the normal human bladder is vital to understanding the cellular origins of benign bladder disease and bladder cancer.
We used single-cell RNA sequencing (scRNA-seq) of 12,423 cells from healthy human bladder tissue samples taken from patients with bladder cancer and 12,884 cells from mouse bladders to classify bladder cell types and their underlying functions.
We created a single-cell transcriptomic map of human and mouse bladders, including 16 clusters of human bladder cells and 15 clusters of mouse bladder cells. The homology and heterogeneity of human and mouse bladder cell types were compared and both conservative and heterogeneous aspects of human and mouse bladder evolution were identified. We also discovered two novel types of human bladder cells. One type is and interstitial cells which may be associated with nerve conduction and allergic reactions. The other type is epithelial cells that may be involved with bladder emptying. We verify these epithelial cells also occur in rat and mouse bladders.
This transcriptomic map provides a resource for studying bladder cell types, specific cell markers, signaling receptors, and genes that will help us to learn more about the relationship between bladder cell types and diseases.
全面了解正常人类膀胱的细胞解剖结构对于理解良性膀胱疾病和膀胱癌的细胞起源至关重要。
我们使用单细胞 RNA 测序（scRNA-seq）对来自膀胱癌患者的 12423 个人类膀胱组织样本和 12884 个小鼠膀胱细胞进行测序，以对膀胱细胞类型及其潜在功能进行分类。
我们创建了人类和小鼠膀胱的单细胞转录组图谱，包括 16 个人类膀胱细胞簇和 15 个小鼠膀胱细胞簇。比较了人和小鼠膀胱细胞类型的同源性和异质性，确定了人和小鼠膀胱进化的保守和异质方面。我们还发现了两种新型的人类膀胱细胞。一种是 和 间充质细胞，可能与神经传导和过敏反应有关。另一种是 上皮细胞，可能与膀胱排空有关。我们验证了这些 上皮细胞也存在于大鼠和小鼠的膀胱中。
该转录组图谱为研究膀胱细胞类型、特定细胞标记物、信号受体和基因提供了资源，有助于我们更多地了解膀胱细胞类型与疾病之间的关系。
