改良牛鲍氏计数板细胞计数法。
Improvement of cell counting method for Neubauer counting chamber.
机构信息
Lab Teaching and Management Center, Chongqing Medical University, Chongqing, China.
College of Pediatrics, Chongqing Medical University, Chongqing, China.
出版信息
J Clin Lab Anal. 2020 Jan;34(1):e23024. doi: 10.1002/jcla.23024. Epub 2019 Aug 30.
BACKGROUND
We compared the cell counting accuracy of the conventional method and the improved method by using Neubauer counting chamber.
METHODS
In the improved method, all the border cells were counted and then divided by two; while, in the conventional method, only border cells on the two boundaries (top and left) were counted.
RESULTS
About 55.814% of the samples showed more accurate results by improved counting method, about 38.372% had more accurate results by conventional counting method, and about 5.814% were counted with similar counting error by both methods. The improved method had significantly smaller counting error than conventional method (P < .05). The distribution ratio of the border cells was an independent factor for counting accuracy (P < .05).
CONCLUSION
Together, the improved counting method can reduce the counting error of the Neubauer counting chamber to some extent, assess the distributing uniformity of border cells, and help to eliminate the samples with large differences in distribution.
背景
我们比较了使用改良纽巴氏计数室和传统方法的细胞计数准确性。
方法
在改良方法中，所有边缘细胞都被计数，然后除以二；而在传统方法中，只计数上下左右两条边界上的边缘细胞。
结果
约 55.814%的样本使用改良计数方法的结果更准确，约 38.372%的样本使用传统计数方法的结果更准确，约 5.814%的样本两种方法的计数误差相似。改良方法的计数误差明显小于传统方法（P<.05）。边缘细胞的分布比例是影响计数准确性的独立因素（P<.05）。
结论
总之，改良计数方法可以在一定程度上减少纽巴氏计数室的计数误差，评估边缘细胞的分布均匀性，有助于排除分布差异较大的样本。
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